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FIGURE: 1 / 1
NDUFA10 Antibody (PA5-87650) in WB
Western blot analysis of NDUFA10 in extracts of various cell lines. Samples were incubated with NDUFA10 Polyclonal antibody (Product # PA5-87650) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-87650
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human NDUFA10 (NP_0045351)
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804319
Product Specific Information
Immunogen sequence: LRYGMWHFLL GDKASKRLTE RSRVITVDGN ICTGKGKLAK EIAEKLGFKH FPEAGIHYPD STTGDGKPLA TDYNGNCSLE KFYDDPRSND GNSYRLQSWL YSSRLLQYSD ALEHLLTTGQ GVVLERSIFS DFVFLEAMYN QGFIRKQCVD HYNEVKSVTI CDYLPPHLVI YIDVPVPEVQ RRIQKKGDPH EMKITSAYLQ DIENAYKKTF LPEMSEKCEV LQYSAREAQD SKKVVEDIEY LKFDKGPWLK QDNRTLYHLR LLVQD; Positive Samples: HepG2, HL-60, BT-474, Jurkat, Mouse liver, Mouse kidney, Rat liver; Cellular Location: Mitochondrion matrix
Target Information
The protein encoded by this gene is a component of 42 kDa complex I, the first enzyme complex in the electron transport chain of mitochondria. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. A mutation in this gene was found in an individual with Leigh syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-42kD; complex I 42kDa subunit; Complex I-42kD; FLJ21923; MGC5103; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH-ubiquinone oxidoreductase 42 kDa subunit
Gene Aliases: 2900053E13Rik; CI-42k; CI-42KD; NDUFA10; ndufa10l
UniProt ID: (Human) O95299, (Mouse) Q99LC3, (Rat) Q561S0
Entrez Gene ID: (Human) 4705, (Mouse) 67273, (Rat) 678759
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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