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FIGURE: 1 / 1
NDUFA12 Antibody (PA5-96981) in WB
Western blot analysis of NDUFA12 in extracts of various cell lines. Samples were incubated with NDUFA12 Polyclonal antibody (Product # PA5-96981) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96981
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human NDUFA12 (NP_0613261)
Conjugate
Form
Liquid
Concentration
2.32 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808783
Product Specific Information
Immunogen sequence: MELVQVLKRG LQQITGHGGL RGYLRVFFRT NDAKVGTLVG EDKYGNKYYE DNKQFFGRHR WVVYTTEMNG KNTFWDVDGS MVPPEWHRWL HSMTDDPPTT KPLTARKFIW TNHKFNVTGT PEQYVPYSTT RKKIQEWIPP STPYK; Positive Samples: 293T, PC-12, HepG2, Mouse kidney, Mouse heart, Mouse testis, Rat heart, Rat brain; Cellular Location: Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Target Information
This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 13 kDa differentiation-associated protein; 13kDa differentiation-associated protein; CI-B17.2; CIB17.2; complex I B17.2 subunit; Complex I-B17.2; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; NADH-ubiquinone oxidoreductase subunit B17.2
Gene Aliases: 2410011G03Rik; AW112974; B17.2; DAP13; NDUFA12; RGD1311462
UniProt ID: (Human) Q9UI09, (Mouse) Q7TMF3
Entrez Gene ID: (Human) 55967, (Rat) 299739, (Mouse) 66414
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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