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Invitrogen
NDUFA4 Polyclonal Antibody
FIGURE: 1 / 3
NDUFA4 Antibody (PA5-51021) in ICC/IF
Immunofluorescence analysis of NDUFA4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NDUFA4 Rabbit Polyclonal Antibody (Product # PA5-51021) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) ... View More
Product Details
PA5-51021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from the C-terminal region of human NDUFA4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2636469
Product Specific Information
NDUFA4 Polyclonal Antibody detects endogenous levels of NDUFA4 protein.
Target Information
Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance. {ECO:0000269; PubMed:22902835}.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CI-MLRQ; Complex I 9kDa subunit; Complex I-MLRQ; Cytochrome c oxidase subunit NDUFA4; EC 1.6.5.3; EC 1.6.99.3; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4 (9 kDa, MLRQ); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4; NADH-ubiquinone oxidoreductase MLRQ subunit
Gene Aliases: CI-9k; CI-MLRQ; MLRQ; NDUFA4
UniProt ID: (Human) O00483, (Mouse) Q62425
Entrez Gene ID: (Human) 4697, (Rat) 681024, (Mouse) 17992
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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