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Invitrogen
NDUFA6 Polyclonal Antibody
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NDUFA6 Antibody (PA5-97259) in WB
Western blot analysis of NDUFA6 in extracts of various cell lines. Samples were incubated with NDUFA6 Polyclonal antibody (Product # PA5-97259) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-97259
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 35-154 of human NDUFA6 (NP_0024812)
Conjugate
Form
Liquid
Concentration
0.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809061
Product Specific Information
Immunogen sequence: ATSTASTFVK PIFSRDMNEA KRRVRELYRA WYREVPNTVH QFQLDITVKM GRDKVREMFM KNAHVTDPRV VDLLVIKGKI ELEETIKVWK QRTHVMRFFH ETEAPRPKDF LSKFYVGHDP; Positive Samples: Raji, HepG2, 22Rv1, Mouse kidney, Mouse heart, Rat kidney; Cellular Location: Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Target Information
NDUFA6 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6), also known as LYRM6 (LYR motif-containing protein 6), NADHB14 or B14, is a 128 amino acid protein that localizes to the matrix side of the mitochondrial membrane and belongs to the complex I LYR family. Existing as a subunit of the multi-protein membrane respiratory chain NADH dehydrogenase complex (Complex I), NDUFA6 functions as an accessory protein that facilitates the transfer of electrons from NADH to the respiratory chain. The gene encoding NDUFA6 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-B14; complex I B14 subunit; Complex I-B14; LYR motif-containing protein 6; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 6; NADH-ubiquinone oxidoreductase B14 subunit
Gene Aliases: 14kDa; 2700038D15Rik; B14; B230217P19Rik; CI-B14; LYRM6; NADHB14; NDUFA6
UniProt ID: (Human) P56556, (Mouse) Q9CQZ5
Entrez Gene ID: (Human) 4700, (Mouse) 67130, (Rat) 315167
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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