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Invitrogen
NDUFAF1 Polyclonal Antibody
FIGURE: 1 / 7
NDUFAF1 Antibody (PA5-59051) in ICC/IF
Immunofluorescence analysis of Complex I intermediate-associated protein 30, mitochondrial was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NDUFAF1 Polyclonal Antibody (Product # PA5-59051) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Seconda... View More
Product Details
PA5-59051
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NDUFAF1. Recombinant protein control fragment (Product #RP-98082).
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2644553
Product Specific Information
Immunogen sequence: LLKDEIVDHW RGPEGHPLHE VLLEQAKVVW QFRGKEDLDK WTVTSDKTIG GRSEVFLKMG KNNQSALLYG TLSSEAPQDG ESTRSGYCAM ISRIPRGAF
Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 84%.
Target Information
The NDUFAF1 gene encodes the human homolog of a Neurospora crassa Cia30 protein involved in the assembly of complex I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Complex I intermediate-associated protein 30, mitochondrial; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1; NADH dehydrogenase (ubiquinone) complex I, assembly factor 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, assembly factor 1
Gene Aliases: CGI-65; CGI65; CIA30; NDUFAF1
UniProt ID: (Human) Q9Y375
Entrez Gene ID: (Human) 51103
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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