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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • NDUFAF5 Antibodies

          Invitrogen

          NDUFAF5 Polyclonal Antibody

          1 Reference
          View all (10) NDUFAF5 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
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          Cite NDUFAF5 Polyclonal Antibody

          • Antibody Testing Data (3)
          NDUFAF5 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          NDUFAF5 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          NDUFAF5 Antibody (PA5-31396) in ICC/IF

          NDUFAF5 Polyclonal Antibody detects C20orf7 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min. Green: C20orf7 protein stained by NDUFAF5 Polyclonal Antibody (Product # PA5-31396) diluted at 1:500. Blue: Hoechst 33342 staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          NDUFAF5 Antibody in Immunocytochemistry (ICC/IF)
          NDUFAF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NDUFAF5 Antibody in Western Blot (WB)

          Product Details

          PA5-31396

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human C20orf7. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.84 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2548870

          Product Specific Information

          Recommended positive controls: NT2D1, IMR32.

          Predicted reactivity: Mouse (93%), Rat (91%), Chicken (83%), Bovine (96%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Arginine-hydroxylase NDUFAF5, mitochondrial; NADH dehydrogenase (ubiquinone) complex I, assembly factor 5; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; probable methyltransferase C20orf7, mitochondrial; Putative methyltransferase NDUFAF5

          View more View less

          Gene Aliases: bA526K24.2; C20orf7; dJ842G6.1; NDUFAF5

          View more View less

          UniProt ID: (Human) Q5TEU4

          View more View less

          Entrez Gene ID: (Human) 79133

          View more View less

          Function(s)
          molecular_function methyltransferase activity
          Process(es)
          methylation mitochondrial respiratory chain complex I assembly
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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