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OriGene
NDUFB3 Monoclonal Antibody (OTI2G5), TrueMAB™
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FIGURE: 1 / 1
NDUFB3 Antibody (CF810650) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB3 (RC201988, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB. Positive lysates LY419291 (100 µg) and LC419291 (20 µg) can be purchased separately from OriGene.
Product Details
CF810650
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2G5
Immunogen
Human recombinant protein fragment corresponding to amino acids 2-98 of human NDUFB3 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-B12; Complex I-B12; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; NADH-ubiquinone oxidoreductase B12 subunit
Gene Aliases: B12; CI-B12; NDUFB3
UniProt ID: (Human) O43676
Entrez Gene ID: (Human) 4709
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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