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NECAP1 Antibody (PA5-100636) in ICC/IF
Immunocytochemistry analysis of NECAP1 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NECAP1 polyclonal antibody (Product # PA5-100636) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100636
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NECAP1(Accession Q8NC96), corresponding to amino acid residues E8-I58.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850142
Product Specific Information
Antibody detects endogenous levels of total NECAP1.
Target Information
Involved in endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adaptin ear-binding clathrin-associated protein; Adaptin ear-binding coat-associated protein 1; adaptin-ear-binding coat-associated protein 1; brain enriched NECAP2 similar protein; clathrin-coated vesicle coat protein; NECAP endocytosis-associated protein 1; NECAP-1; snoRNA MBI-129
Gene Aliases: 1200016B17Rik; AI747569; EIEE21; mFLJ00061; NECAP1; RGD1306053
UniProt ID: (Human) Q8NC96, (Rat) P69682, (Mouse) Q9CR95
Entrez Gene ID: (Human) 25977, (Rat) 312694, (Mouse) 67602
Performance Guarantee
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