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NEDD4L Monoclonal Antibody (2F6H6), CoraLite® Plus 488

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

CL488-67276

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.4 µg/1x10^6 cell suspension
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F6H6

Immunogen

Recombinant protein
View immunogen

Conjugate

CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488

Excitation/Emission Max

493/522 nm

Form

Liquid

Purification

Protein G

Storage buffer

PBS with 0.5% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, store in dark

Shipping conditions

Wet ice

Product Specific Information

Immunogen sequence: EESYRRIMSV KRPDVLKARL WIEFESEKGL DYGGVAREWF FLLSKEMFNP YYGLFEYSAT DNYTLQINPN SGLCNEDHLS YFTFIGRVAG LAVFHGKLLD GFFIRPFYKM MLGKQITLND MESVDSEYYN SLKWILENDP TELDLMFCID EENFGQTYQV DLKPNGSEIM VTNENKREYI DLVIQWRFVN RVQKQMNAFL EGFTELLPID LIKIFDENEL ELLMCGLGDV DVNDWRQHSI YKNGYCPNHP VIQWFWKAVL LMDAEKRIRL LQFVTGTSRV PMNGFAELYG SNGPQLFTIE QWGSPEKLPR AHTCFNRLDL PPYETFEDLR EKLLMAVENA QGFEGVD

Target Information

NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase NEDD4-like; FLJ33870; HECT-type E3 ubiquitin transferase NED4L; Nedd4-2; NEDD4.2; neural precursor cell expressed, developmentally down-regulated gene 4b; ubiquitin-protein ligase Rsp5

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Gene Aliases: 1300012C07Rik; hNEDD4-2; KIAA0439; NEDD4-2; NEDD4.2; Nedd4b; NEDD4L; NEDL3; RSP5

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UniProt ID: (Human) Q96PU5

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Entrez Gene ID: (Human) 23327, (Rat) 291553, (Mouse) 83814

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Function(s)
ubiquitin-protein transferase activity protein binding potassium channel regulator activity ligase activity sodium channel regulator activity potassium channel inhibitor activity sodium channel inhibitor activity ion channel binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination regulation of membrane depolarization protein monoubiquitination sodium ion transport cellular sodium ion homeostasis excretion response to metal ion protein ubiquitination viral life cycle water homeostasis ion transmembrane transport regulation of ion transmembrane transport regulation of protein catabolic process regulation of membrane potential protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of protein catabolic process positive regulation of endocytosis regulation of membrane repolarization protein K48-linked ubiquitination ventricular cardiac muscle cell action potential regulation of potassium ion transmembrane transporter activity negative regulation of potassium ion transmembrane transporter activity negative regulation of potassium ion transmembrane transport negative regulation of sodium ion transmembrane transport positive regulation of dendrite extension negative regulation of protein localization to cell surface negative regulation of sodium ion transmembrane transporter activity positive regulation of caveolin-mediated endocytosis negative regulation of systemic arterial blood pressure response to salt stress positive regulation of sodium ion transport negative regulation of sodium ion transport ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway regulation of bicellular tight junction assembly positive regulation of cation channel activity
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