Invitrogen

NEDD8 Polyclonal Antibody

Advanced Verification

17 Published Figures

7 References

View all (20) NEDD8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (17)
Advanced Verification (2)

FIGURE: 1 / 19

NEDD8 Antibody (34-1400) in WB

Nature communications 2023 - Fig. 6 NLRX1-mediated ROS controls NF-kappaB signalling and affects cullin 1 NEDDylation. Immunofluorescence microscopy of A549 cells stained with antibody for the p65 NF-kappaB subunit. Cells were pre-treated with mitoTEMPO (10 muM, 2 h pre-infection), or with vehicle control (DMSO) and infected with Kp52145 for 3 h ( a ). b Cells were transfected with NLRX1 siRNA (50 nM) or a non-silencing control (AS) an... View More

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NEDD8 Antibody — Fig. 4 IL-1beta treatment of primary neuronal cultures leads to NEDD8 translocation. Fluorescent immunocytochemistry of representative cultures of untreated ( a ) and IL-1beta-treated ( b ) primary rat neurons ( n = 6 each) depict greater translocation of NEDD8 (green) from nucleus (blue) to cytoplasm when IL-1beta is present, ** p < 0.01, ++ p < 0.01 ( c ). Quantification of western immunoblots ( d ) of nuclear (histone) and cytoplasmic (actin) cell fractions from untreated and IL-1beta-treated cultures ( n = 6 separate experiments) demonstrates the susceptibility of NEDD8 to undergo nuclear to cytoplasmic translocation in the presence of excess IL-1beta, ** p < 0.01, *** p < 0.01 ( e ). Quantification of NEDD8/parkin colocation (<= 40 nm) in untreated NT2 cells ( f ) compared with IL-1beta-treated NT2 cells ( g ) provides evidence of IL-1beta-induced interaction between of parkin and NEDD8 in NT2 cells, two-tailed t test, * p < 0.05 ( h ). NEDD8-parkin proximity (red dots) was quantified with FIJI (ImageJ) Particle Analysis {TM}

Product Details

34-1400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Insect

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the N-terminal sequence of human NEDD8

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533155

Target Information

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infection.

Nature communications

Sá-Pessoa J,López-Montesino S,Przybyszewska K,Rodríguez-Escudero I,Marshall H,Ova A,Schroeder GN,Barabas P,Molina M,Curtis T,Cid VJ,Bengoechea JA

Published figure using NEDD8 polyclonal antibody (Product # 34-1400) in Western Blot

Thu Feb 16 00:00:00 UTC 2023

COP9-Signalosome deneddylase activity is enhanced by simultaneous neddylation: insights into the regulation of an enzymatic protein complex.

Cell division

Bornstein G,Grossman C

34-1400 was used in western blot to study the effect of neddylation on COP9-Signalosome deneddylase activity

Wed Aug 12 00:00:00 UTC 2015

The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics.

The Journal of biological chemistry

Toro TB,Toth JI,Petroski MD

34-1400 was used in western blot to elucidate how cycle inhibiting factor modulates NEDD8-dependent signaling.

Fri May 24 00:00:00 UTC 2013

The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics.

The Journal of biological chemistry

Toro TB,Toth JI,Petroski MD

34-1400 was used in western blot to elucidate how cycle inhibiting factor modulates NEDD8-dependent signaling.

Fri May 24 00:00:00 UTC 2013

A gatekeeper residue for NEDD8-activating enzyme inhibition by MLN4924.

Cell reports

Toth JI,Yang L,Dahl R,Petroski MD

34-1400 was used in western blot to elucidate resistance to MLN4924.

Thu Apr 19 00:00:00 UTC 2012

UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1α accumulation.

BMC biology

Bandau S,Knebel A,Gage ZO,Wood NT,Alexandru G

Thu Apr 26 00:00:00 UTC 2012

Development and validation of a method for profiling post-translational modification activities using protein microarrays.

PloS one

Del Rincón SV,Rogers J,Widschwendter M,Sun D,Sieburg HB,Spruck C

34-1400 was used in western blot to generate a protein microarray-based assay to measure post-translational modifications

Mon Jun 28 00:00:00 UTC 2010

Development and validation of a method for profiling post-translational modification activities using protein microarrays.

PloS one

Del Rincón SV,Rogers J,Widschwendter M,Sun D,Sieburg HB,Spruck C

34-1400 was used in western blot to generate a protein microarray-based assay to measure post-translational modifications

Mon Jun 28 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; Ubiquitin-like protein NEDD8

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Gene Aliases: NEDD-8; NEDD8

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UniProt ID: (Human) Q6LES6

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Entrez Gene ID: (Human) 4738

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Function(s)

protein binding ubiquitin protein ligase binding

Process(es)

regulation of transcription from RNA polymerase II promoter cellular protein modification process proteolysis ubiquitin-dependent protein catabolic process transforming growth factor beta receptor signaling pathway protein localization anatomical structure morphogenesis response to organic cyclic compound protein neddylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NEDD8 Polyclonal Antibody

NEDD8 Antibody (34-1400) in WB

Product Details

34-1400

Western Blot (WB)

ELISA (ELISA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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