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Invitrogen
NEDD8 Polyclonal Antibody
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FIGURE: 1 / 6
NEDD8 Antibody (PA5-95403) in ICC/IF
Immunocytochemistry analysis of NEDD8 using anti-NEDD8 antibody (Product # PA5-95403) . NEDD8 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-NEDD8 antibody (Product # PA5-95403) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. V... View More
Product Details
PA5-95403
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NEDD8 (20-60aa TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807206
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human MCF-7 whole cell, human RT4 whole cell, human PC-3 whole cell, rat brain tissue, rat thymus tissue, mouse brain tissue, mouse thymus tissue. IHC: human lung cancer tissue. ICC/IF: A431 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NEDD-8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated gene 8; Ubiquitin-like protein NEDD8
Gene Aliases: NEDD-8; NEDD8; Rub1
UniProt ID: (Human) Q6LES6, (Rat) Q71UE8, (Mouse) P29595
Entrez Gene ID: (Human) 4738, (Rat) 25490, (Mouse) 18002
regulation of transcription from RNA polymerase II promoter
protein localization
response to organic cyclic compound
protein neddylation
cellular protein modification process
proteolysis
ubiquitin-dependent protein catabolic process
transforming growth factor beta receptor signaling pathway
anatomical structure morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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