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Invitrogen
NEDD8 Monoclonal Antibody (B.226.2)
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FIGURE: 1 / 5
NEDD8 Antibody (MA5-15028) in ICC/IF
Immunofluorescence analysis of NEDD8 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NEDD8 Monoclonal Antibody (B.226.2) (Product # MA5-15028) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conj... View More
Product Details
MA5-15028
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
B.226.2
Immunogen
Synthetic peptide corresponding to amino acids at the amino terminus of human NEDD8 protein
Conjugate
Form
Liquid
Concentration
114 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10985618
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 and ISG15.
Target Information
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NEDD-8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated gene 8; Ubiquitin-like protein NEDD8
Gene Aliases: NEDD-8; NEDD8; Rub1
UniProt ID: (Human) Q6LES6, (Rat) Q71UE8, (Mouse) P29595
Entrez Gene ID: (Human) 4738, (Rat) 25490, (Mouse) 18002
regulation of transcription from RNA polymerase II promoter
protein localization
response to organic cyclic compound
protein neddylation
cellular protein modification process
proteolysis
ubiquitin-dependent protein catabolic process
transforming growth factor beta receptor signaling pathway
anatomical structure morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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