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Invitrogen
NEFL Monoclonal Antibody (7D1)
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NEFL Antibody (MA5-47467) in IHC (PFA)
Immunohistochemistry analysis of NEFL in section of mouse cerebellum. Samples were stained with NEFL monoclonal antibody (Product # MA5-47467) using a dilution of 1:5,000 (green). Costained with chicken pAb to FOX2 (Product # PA5-143566) dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours,... View More
Product Details
MA5-47467
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
7D1
Immunogen
Full length native protein purified from pig spinal cord
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942449
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; BOS_9100; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Bovine) P02548, (Human) P07196, (Mouse) P08551, (Rat) P19527
Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613
microtubule cytoskeleton organization
anterograde axonal transport
retrograde axonal transport
peripheral nervous system axon regeneration
axonal transport of mitochondrion
regulation of axon diameter
neurofilament bundle assembly
locomotion
negative regulation of neuron apoptotic process
intermediate filament organization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
neurofilament cytoskeleton organization
MAPK cascade
response to toxic substance
spinal cord development
hippocampus development
cerebral cortex development
response to peptide hormone
positive regulation of GTPase activity
intermediate filament polymerization or depolymerization
protein polymerization
response to corticosterone
response to sodium arsenite
response to acrylamide
response to organic substance
response to organonitrogen compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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