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Invitrogen
NEFM Polyclonal Antibody
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NEFM Antibody (PA5-85777) in ICC/IF
NEFM Polyclonal Antibody detects NF-M protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-M protein stained byNEFM Polyclonal Antibody (Product # PA5-85777) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta-3 Tubulin Polyclonal Antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-85777
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse NF-M. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792913
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Human (97%), Mouse (100%), Rat (100%), Xenopus laevis (89%), Chicken (90%), Bovine (97%).
Positive Control: mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament-M; NF-M; NMC
Gene Aliases: Nef3; Nefm; NF-M; NF160; NF165; Nfm
UniProt ID: (Mouse) P08553, (Rat) P12839
Entrez Gene ID: (Mouse) 18040, (Rat) 24588
microtubule cytoskeleton organization
axo-dendritic transport
regulation of axon diameter
neurofilament bundle assembly
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
spinal cord development
axon regeneration
cellular response to oxidative stress
cellular response to estradiol stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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