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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • NEFM Antibodies

          Invitrogen

          NEFM Monoclonal Antibody (RMO-270)

          Advanced Verification
          16 Published Figures
          60 References
          View all (35) NEFM antibodies

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          Datasheet
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          Datasheet
          Protocols
          Questions & Answers

          Cite NEFM Monoclonal Antibody (RMO-270)

          • Antibody Testing Data (7)
          • Published Figures (16)
          • Advanced Verification (3)
          NEFM Antibody in Immunocytochemistry (ICC/IF)
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          NEFM Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 26

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          NEFM Antibody (13-0700) in ICC/IF

          Immunofluorescence analysis of NF-M was performed using 70% confluent log phase HEK-293 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. HEK-293 cells were labeled with NF-M Mouse Monoclonal Antibody (Product # 13-0700) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          NEFM Antibody in Immunocytochemistry (ICC/IF)
          NEFM Antibody in Immunocytochemistry (ICC/IF)
          NEFM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NEFM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NEFM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NEFM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NEFM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NEFM Antibody in Immunocytochemistry (ICC/IF)
          NEFM Antibody in Immunocytochemistry (ICC/IF)
          NEFM Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          NEFM Antibody in Immunohistochemistry (Frozen) (IHC (F))
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Immunohistochemistry (IHC)
          NEFM Antibody in Flow Cytometry (Flow)
          NEFM Antibody
          NEFM Antibody
          NEFM Antibody

          Product Details

          13-0700

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5-1 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 29 publications 29 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          View 3 publications 3 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 15 publications 15 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -

          Immunoprecipitation (IP)

          2-5 µg
          -

          Functional Assay (Functional)

          -
          View 1 publication 1 publication

          In vitro Assay (IV)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 7 publications 7 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Avian, Chicken, Human, Mouse, Rat, Xenopus, Zebrafish

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          RMO-270

          Immunogen

          Rat neurofilaments
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2532998

          Product Specific Information

          The cloning partner for this antibody is Sp/2.

          This antibody reacts with the 160 kD polypeptide subunit of human neurofilament. It specifically recognizes a phosphate-independent epitope in the tail (carboxy) domain of NF-M of most vertebrates and invertebrates.

          This antibody is suitable for immunohistochemical staining of Bouin's and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.

          Target Information

          Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 160 kDa neurofilament protein; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NMC

          View more View less

          Gene Aliases: NEF3; NEFM; NF-M; NF160; NF165; NFM

          View more View less

          UniProt ID: (Human) P07197, (Mouse) P08553, (Rat) P12839

          View more View less

          Entrez Gene ID: (Human) 4741, (Mouse) 18040, (Rat) 24588

          View more View less

          Function(s)
          structural constituent of cytoskeleton protein binding microtubule binding receptor binding structural molecule activity toxic substance binding protein heterodimerization activity
          Process(es)
          neurofilament bundle assembly axon development mitophagy in response to mitochondrial depolarization microtubule cytoskeleton organization axo-dendritic transport regulation of axon diameter intermediate filament cytoskeleton organization intermediate filament polymerization or depolymerization intermediate filament bundle assembly neurofilament cytoskeleton organization spinal cord development axon regeneration cellular response to oxidative stress cellular response to estradiol stimulus
          It has to be done as per old AB suggested Products section.
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