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          • Primary Antibodies ›
          • NEK11 Antibodies

          Bioss

          NEK11 Polyclonal Antibody

          View all (15) NEK11 antibodies

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          Cite NEK11 Polyclonal Antibody

          • Antibody Testing Data (3)
          NEK11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          NEK11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          NEK11 Antibody (BS-19195R) in IHC (P)

          Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
          NEK11 Polyclonal Antibody, Unconjugated (bs-19195R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for ... View More
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          NEK11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NEK11 Antibody in Western Blot (WB)
          NEK11 Antibody in Western Blot (WB)

          Product Details

          BS-19195R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:300
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:400
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic peptide derived from human NEK11, amino acids 151-250.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          NEK11 (FLJ23495) belongs to the NIMA family of kinases which are involved in DNA replication and genotoxic stress response. NEK11 encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: nek11; Never in mitosis A-related kinase 11; NIMA (never in mitosis gene a)- related kinase 11; NIMA (never in mitosis gene a)-related expressed kinase 11; NIMA-related kinase 11; NimA-related protein kinase 11; Serine/threonine-protein kinase Nek11

          View more View less

          Gene Aliases: 4932416N14Rik; NEK11

          View more View less

          UniProt ID: (Human) Q8NG66, (Mouse) Q8C0Q4

          View more View less

          Entrez Gene ID: (Human) 79858, (Rat) 315978, (Mouse) 208583

          View more View less

          Function(s)
          protein serine/threonine kinase activity protein binding ATP binding metal ion binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
          Process(es)
          protein phosphorylation intra-S DNA damage checkpoint intracellular signal transduction cell cycle phosphorylation histone phosphorylation G2 DNA damage checkpoint mitotic cell cycle phase transition regulation of mitotic cell cycle phase transition
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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