Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
NEK2 Antibody (600-401-452) in WB
Western Blot of Rabbit Anti-NEK2 Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10µg) (p/n W09-001-370). Primary Antibody: Anti-NEK2 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30mins at RT. Block:... View More
Product Details
600-401-452
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee. Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
Target Information
NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: HSPK 21; NEK 2; NEK 2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NIMA-related kinase 2; NimA-related protein kinase 2; NLK 1; protein phosphatase 1, regulatory subunit 111; Serine/threonine-protein kinase Nek2
Gene Aliases: HsPK21; NEK2; NEK2A; NLK1; PPP1R111; RP67
UniProt ID: (Human) P51955
Entrez Gene ID: (Human) 4751
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
blastocyst development
protein phosphorylation
chromosome segregation
mitotic nuclear division
regulation of mitotic nuclear division
positive regulation of telomere maintenance via telomerase
negative regulation of DNA binding
regulation of mitotic centrosome separation
protein autophosphorylation
spindle assembly
centrosome separation
cell division
meiotic cell cycle
positive regulation of telomerase activity
regulation of attachment of spindle microtubules to kinetochore
mitotic spindle assembly
negative regulation of centriole-centriole cohesion
positive regulation of telomere capping
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support