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NEK2 Antibody (MA5-50807) in WB
Western blot analysis of NEK2 in different various lysates. Lane 1: A549 cell lysate; Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa. Observed band size: 52 kDa. Exposure time: 2 minutes; 10% SDS-PAGE gel. Primary antibody NEK2 recombinant monoclonal antibody (Product # MA5-50807) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:200,000 was used for 1 hour at room temperature. Proteins were transferred ... View More
Product Details
MA5-50807
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE37-74
Immunogen
Synthetic peptide within Human NEK2 aa 1-50/445.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093104
Product Specific Information
Positive control: A549 cell lysate, Hela cell lysate.
Predicted band size: 52 kDa
Subcellular Location: Nucleus, nucleolus, Cytoplasm.
Target Information
NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HSPK 21; NEK 2; NEK 2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NIMA-related kinase 2; NimA-related protein kinase 2; NLK 1; protein phosphatase 1, regulatory subunit 111; Serine/threonine-protein kinase Nek2
Gene Aliases: HsPK21; NEK2; NEK2A; NLK1; PPP1R111; RP67
UniProt ID: (Human) P51955
Entrez Gene ID: (Human) 4751
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
blastocyst development
protein phosphorylation
chromosome segregation
mitotic nuclear division
regulation of mitotic nuclear division
positive regulation of telomere maintenance via telomerase
negative regulation of DNA binding
regulation of mitotic centrosome separation
protein autophosphorylation
spindle assembly
centrosome separation
cell division
meiotic cell cycle
positive regulation of telomerase activity
regulation of attachment of spindle microtubules to kinetochore
mitotic spindle assembly
negative regulation of centriole-centriole cohesion
positive regulation of telomere capping
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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