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NEK4 Antibody (PA5-104126) in IHC (P)
Immunohistochemistry analysis of NEK4 in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NEK4 polyclonal antibody (Product # PA5-104126) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-104126
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NEK4(Accession P51957), corresponding to amino acid residues H499-E549.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853454
Product Specific Information
Antibody detects endogenous levels of total NEK4.
Target Information
NEK4 is a serine/threonine kinase involved in signal transduction and cellular communication. It is proposed to act exclusively upon threonine residues and is present in most primary carcinomas. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Never in mitosis A-related kinase 4; NIMA (never in mitosis gene a)-related expressed kinase 4; NIMA (never in mitosis gene a)-related kinase 4; NIMA-related kinase 4; NimA-related protein kinase 4; serine/threonine kinase 2; serine/threonine protein kinase-2; Serine/threonine-protein kinase 2; Serine/threonine-protein kinase Nek4; serine/threonine-protein kinase Nek4 variant 2; Serine/threonine-protein kinase NRK2
Gene Aliases: NEK4; NRK2; pp12301; STK2
UniProt ID: (Human) P51957, (Mouse) Q9Z1J2
Entrez Gene ID: (Human) 6787, (Mouse) 23955, (Rat) 306252
mitotic cell cycle
protein phosphorylation
cellular response to DNA damage stimulus
mitotic nuclear division
signal transduction by protein phosphorylation
positive regulation of transcription, DNA-templated
cell division
regulation of replicative cell aging
regulation of cellular senescence
regulation of response to DNA damage stimulus
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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