Proteintech

NELFE Polyclonal Antibody

NELFE Antibody in Immunocytochemistry (ICC/IF)

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NELFE Antibody (10705-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10705-1-AP (NELFE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NELFE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NELFE Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10705-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:12,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

Assay-dependent

View 4 publications 4 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NELFE Fusion Protein Ag1186 (1-333 aa encoded by BC011600)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

10705-1-AP is obtained by immunizing animals with full-length RDBP of human origin.

Immunogen sequence: MLVIPPGLS EEEEALQKKF NKLKKKKKAL LALKKQSSSS TTSQGGVKRS LSEQPVMDTA TATEQAKQLV KSGAISAIKA ETKNSGFKRS RTLEGKLKDP EKGPVPTFQP FQRSISADDD LQESSRRPQR KSLYESDRLR ELGPDGEEAE GPGAGDGPPR SFDWGYEERS GAHSSASPPR SRSRDRSHER NRDRDREGPF RRSDSFPERR APRKGNTLYV YGEDMTPTLL RGAFSPFGNI IDLSMDPPRN CAFVTYEKME SADQAVAELN GTQVESVQLK VNIARKQPML DAATGKSVWG SLAVQNSPKG CHRDKRTQIV YSDDVYKENL VDGF (1-333 aa encoded by BC011600)

Target Information

RDBP is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.

Mucosal immunology

Ou J,Guan X,Wang J,Wang T,Zhang B,Li R,Xu H,Hu X,Guo XK

Tue Feb 01 00:00:00 UTC 2022

Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve.

PLoS genetics

Chignon A,Argaud D,Boulanger MC,Mkannez G,Bon-Baret V,Li Z,Thériault S,Bossé Y,Mathieu P

Sat Jan 01 00:00:00 UTC 2022

Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve.

PLoS genetics

Chignon A,Argaud D,Boulanger MC,Mkannez G,Bon-Baret V,Li Z,Thériault S,Bossé Y,Mathieu P

Sat Jan 01 00:00:00 UTC 2022

Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve.

PLoS genetics

Chignon A,Argaud D,Boulanger MC,Mkannez G,Bon-Baret V,Li Z,Thériault S,Bossé Y,Mathieu P

Sat Jan 01 00:00:00 UTC 2022

RNA polymerase II pausing factor NELF in CD8⁺ T cells promotes antitumor immunity.

Nature communications

Wu B,Zhang X,Chiang HC,Pan H,Yuan B,Mitra P,Qi L,Simonyan H,Young CN,Yvon E,Hu Y,Zhang N,Li R

Wed Apr 20 00:00:00 UTC 2022

Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag.

STAR protocols

Li Y,Nakka K,Olender T,Gingras-Gelinas P,Wong MM,Robinson DCL,Bandukwala H,Palii CG,Neyret O,Brand M,Blais A,Dilworth FJ

Fri Sep 17 00:00:00 UTC 2021

Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.

Developmental cell

Robinson DCL,Ritso M,Nelson GM,Mokhtari Z,Nakka K,Bandukwala H,Goldman SR,Park PJ,Mounier R,Chazaud B,Brand M,Rudnicki MA,Adelman K,Dilworth FJ

Mon Apr 05 00:00:00 UTC 2021

The RNA-Binding Protein NELFE Promotes Gastric Cancer Growth and Metastasis Through E2F2.

Frontiers in oncology

Chen C,Zheng Q,Pan S,Chen W,Huang J,Cao Y,Tu Y,Li Z,Yu C,Jie Z

Sat Jul 24 00:00:00 UTC 2021

The RNA-Binding Protein NELFE Promotes Gastric Cancer Growth and Metastasis Through E2F2.

Frontiers in oncology

Chen C,Zheng Q,Pan S,Chen W,Huang J,Cao Y,Tu Y,Li Z,Yu C,Jie Z

Sat Jul 24 00:00:00 UTC 2021

Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.

Nature communications

Yu L,Zhang B,Deochand D,Sacta MA,Coppo M,Shang Y,Guo Z,Zeng X,Rollins DA,Tharmalingam B,Li R,Chinenov Y,Rogatsky I,Hu X

Fri May 08 00:00:00 UTC 2020

Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.

Nature communications

Yu L,Zhang B,Deochand D,Sacta MA,Coppo M,Shang Y,Guo Z,Zeng X,Rollins DA,Tharmalingam B,Li R,Chinenov Y,Rogatsky I,Hu X

Fri May 08 00:00:00 UTC 2020

The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Nature

Liu B,Xu Q,Wang Q,Feng S,Lai F,Wang P,Zheng F,Xiang Y,Wu J,Nie J,Qiu C,Xia W,Li L,Yu G,Lin Z,Xu K,Xiong Z,Kong F,Liu L,Huang C,Yu Y,Na J,Xie W

Sun Nov 01 00:00:00 UTC 2020

Negative elongation factor is essential for endometrial function.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hewitt SC,Li R,Adams N,Winuthayanon W,Hamilton KJ,Donoghue LJ,Lierz SL,Garcia M,Lydon JP,DeMayo FJ,Adelman K,Korach KS

Fri Feb 01 00:00:00 UTC 2019

Negative elongation factor is essential for endometrial function.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hewitt SC,Li R,Adams N,Winuthayanon W,Hamilton KJ,Donoghue LJ,Lierz SL,Garcia M,Lydon JP,DeMayo FJ,Adelman K,Korach KS

Fri Feb 01 00:00:00 UTC 2019

Negative elongation factor is essential for endometrial function.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hewitt SC,Li R,Adams N,Winuthayanon W,Hamilton KJ,Donoghue LJ,Lierz SL,Garcia M,Lydon JP,DeMayo FJ,Adelman K,Korach KS

Fri Feb 01 00:00:00 UTC 2019

Negative elongation factor is essential for endometrial function.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hewitt SC,Li R,Adams N,Winuthayanon W,Hamilton KJ,Donoghue LJ,Lierz SL,Garcia M,Lydon JP,DeMayo FJ,Adelman K,Korach KS

Fri Feb 01 00:00:00 UTC 2019

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

eLife

Sacta MA,Tharmalingam B,Coppo M,Rollins DA,Deochand DK,Benjamin B,Yu L,Zhang B,Hu X,Li R,Chinenov Y,Rogatsky I

Fri Feb 09 00:00:00 UTC 2018

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

eLife

Sacta MA,Tharmalingam B,Coppo M,Rollins DA,Deochand DK,Benjamin B,Yu L,Zhang B,Hu X,Li R,Chinenov Y,Rogatsky I

Fri Feb 09 00:00:00 UTC 2018

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

eLife

Sacta MA,Tharmalingam B,Coppo M,Rollins DA,Deochand DK,Benjamin B,Yu L,Zhang B,Hu X,Li R,Chinenov Y,Rogatsky I

Fri Feb 09 00:00:00 UTC 2018

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

eLife

Sacta MA,Tharmalingam B,Coppo M,Rollins DA,Deochand DK,Benjamin B,Yu L,Zhang B,Hu X,Li R,Chinenov Y,Rogatsky I

Fri Feb 09 00:00:00 UTC 2018

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Nucleic acids research

Lu X,Zhu X,Li Y,Liu M,Yu B,Wang Y,Rao M,Yang H,Zhou K,Wang Y,Chen Y,Chen M,Zhuang S,Chen LF,Liu R,Chen R

Fri Aug 19 00:00:00 UTC 2016

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Nucleic acids research

Lu X,Zhu X,Li Y,Liu M,Yu B,Wang Y,Rao M,Yang H,Zhou K,Wang Y,Chen Y,Chen M,Zhuang S,Chen LF,Liu R,Chen R

Fri Aug 19 00:00:00 UTC 2016

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.

Neurobiology of disease

Bastide MF,Bido S,Duteil N,Bézard E

Fri Jan 01 00:00:00 UTC 2016

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.

Neurobiology of disease

Bastide MF,Bido S,Duteil N,Bézard E

Fri Jan 01 00:00:00 UTC 2016

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.

Neurobiology of disease

Bastide MF,Bido S,Duteil N,Bézard E

Fri Jan 01 00:00:00 UTC 2016

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.

Neurobiology of disease

Bastide MF,Bido S,Duteil N,Bézard E

Fri Jan 01 00:00:00 UTC 2016

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Proceedings of the National Academy of Sciences of the United States of America

Gupte R,Muse GW,Chinenov Y,Adelman K,Rogatsky I

Tue Sep 03 00:00:00 UTC 2013

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Proceedings of the National Academy of Sciences of the United States of America

Gupte R,Muse GW,Chinenov Y,Adelman K,Rogatsky I

Tue Sep 03 00:00:00 UTC 2013

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Proceedings of the National Academy of Sciences of the United States of America

Gupte R,Muse GW,Chinenov Y,Adelman K,Rogatsky I

Tue Sep 03 00:00:00 UTC 2013

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Proceedings of the National Academy of Sciences of the United States of America

Gupte R,Muse GW,Chinenov Y,Adelman K,Rogatsky I

Tue Sep 03 00:00:00 UTC 2013

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Cell

Sawarkar R,Sievers C,Paro R

Fri May 11 00:00:00 UTC 2012

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Cell

Sawarkar R,Sievers C,Paro R

Fri May 11 00:00:00 UTC 2012

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Cell

Sawarkar R,Sievers C,Paro R

Fri May 11 00:00:00 UTC 2012

Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.

Oncology reports

Iida M,Iizuka N,Tsunedomi R,Tsutsui M,Yoshida S,Maeda Y,Tokuhisa Y,Sakamoto K,Yoshimura K,Tamesa T,Oka M

Wed Aug 01 00:00:00 UTC 2012

Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.

Oncology reports

Iida M,Iizuka N,Tsunedomi R,Tsutsui M,Yoshida S,Maeda Y,Tokuhisa Y,Sakamoto K,Yoshimura K,Tamesa T,Oka M

Wed Aug 01 00:00:00 UTC 2012

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Proceedings of the National Academy of Sciences of the United States of America

Adelman K,Kennedy MA,Nechaev S,Gilchrist DA,Muse GW,Chinenov Y,Rogatsky I

Tue Oct 27 00:00:00 UTC 2009

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Proceedings of the National Academy of Sciences of the United States of America

Adelman K,Kennedy MA,Nechaev S,Gilchrist DA,Muse GW,Chinenov Y,Rogatsky I

Tue Oct 27 00:00:00 UTC 2009

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Proceedings of the National Academy of Sciences of the United States of America

Adelman K,Kennedy MA,Nechaev S,Gilchrist DA,Muse GW,Chinenov Y,Rogatsky I

Tue Oct 27 00:00:00 UTC 2009

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Proceedings of the National Academy of Sciences of the United States of America

Adelman K,Kennedy MA,Nechaev S,Gilchrist DA,Muse GW,Chinenov Y,Rogatsky I

Tue Oct 27 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: major histocompatibility complex gene RD; Negative elongation factor E; negative elongation factor polypeptide E; NELF E; NELF-E; nuclear protein; RD RNA binding protein; RD RNA-binding protein; RNA-binding protein RD

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Gene Aliases: D17H6S45; D6S45; NELF-E; NELFE; RD; RDBP; RDP

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UniProt ID: (Human) P18615, (Mouse) P19426

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Entrez Gene ID: (Human) 7936, (Mouse) 27632, (Rat) 294258

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Function(s)

nucleotide binding chromatin binding RNA binding protein binding poly(A) RNA binding nucleic acid binding

Process(es)

transcription from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter biological_process negative regulation of transcription elongation from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter positive regulation of viral transcription positive regulation of histone H3-K4 methylation positive regulation of ERK1 and ERK2 cascade negative regulation of mRNA polyadenylation transcription, DNA-templated regulation of transcription, DNA-templated negative regulation of transcription from RNA polymerase II promoter

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NELFE Polyclonal Antibody

NELFE Antibody (10705-1-AP) in ICC/IF

Product Details

10705-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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