OriGene

NF Monoclonal Antibody (OTI3F7), TrueMAB™

View all (2) NEFH/NEFM/NEFL antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF190077

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3F7

Immunogen

Synthetic peptide corresponding to the conserved region of human neurofilament heavy polypeptide (NEFH), medium polypeptide (NEFM) and light polypeptide (NF68, NEFL)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Synthetic peptide sequence is EYQDLLNVKMALDIEIAAYRKLLEGEE

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Diseases associated with NEFM include Pineal Parenchymal Tumor Of Intermediate Differentiation and Wallerian Degeneration. Among its related pathways are Amyotrophic lateral sclerosis (ALS) and Neural Stem Cell Differentiation Pathways and Lineage-specific Markers. Gene Ontology (GO) annotations related to this gene include structural molecule activity and structural constituent of cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 160 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; light molecular weight neurofilament protein; neurofilament; Neurofilament 3; neurofilament heavy; Neurofilament heavy polypeptide; neurofilament light; Neurofilament light polypeptide; Neurofilament medium polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament, heavy polypeptide 200kDa; neurofilament, light polypeptide 68kDa; neurofilament, medium polypeptide 150kDa; neurofilament-3; neurofilament-3 (150 kD medium); neurofilament-l; NF-H; NF-L; NF-M; NHC; NLC; protein phosphatase 1, regulatory subunit 110

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Gene Aliases: CMT1F; CMT2CC; CMT2E; KIAA0845; NEF3; NEFH; NEFL; NEFM; NF-L; NF-M; NF68; NFH; NFL; NFM; PPP1R110

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UniProt ID: (Human) Q9UJS7, (Human) P07196, (Human) P07197

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Entrez Gene ID: (Human) 4744, (Human) 4747, (Human) 4741

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Function(s)
structural molecule activity structural constituent of cytoskeleton microtubule binding toxic substance binding kinesin binding protein kinase binding protein binding, bridging dynein binding protein heterodimerization activity Ras guanyl-nucleotide exchange factor activity protein binding protein C-terminus binding protein domain specific binding identical protein binding phospholipase binding
Process(es)
MAPK cascade microtubule cytoskeleton organization anterograde axonal transport retrograde axonal transport response to toxic substance peripheral nervous system axon regeneration axonal transport of mitochondrion spinal cord development hippocampus development cerebral cortex development regulation of axon diameter neurofilament bundle assembly locomotion response to peptide hormone negative regulation of neuron apoptotic process positive regulation of GTPase activity intermediate filament polymerization or depolymerization intermediate filament organization neuron projection morphogenesis positive regulation of axonogenesis neuromuscular process controlling balance protein polymerization response to corticosterone response to sodium arsenite response to acrylamide ovarian follicle atresia axonogenesis cell projection assembly axon regeneration cellular response to oxidative stress response to cocaine peripheral nervous system neuron axonogenesis axon development cellular response to estradiol stimulus regulation of organelle transport along microtubule mitophagy in response to mitochondrial depolarization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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