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NF-H Monoclonal Antibody (1A3C7)

NF-H Antibody in Immunocytochemistry (ICC/IF)

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NF-H Antibody (60331-1-IG) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 60331-1-IG (NF200 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

NF-H Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

60331-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1A3C7

Immunogen

NF200 Fusion Protein Ag13517 (1-214 aa encoded by BC014185)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognize NEFH only.

Immunogen sequence: MMSFGGADA LLGAPFAPLH GGGSLHYALA RKGGAGGTRS AAGSSSGFHS WTRTSVSSVS ASPSRFRGAG AASSTDSLDT LSNGPEGCMV AVATSRSEKE QLQALNDRFA GYIDKVRQLE AHNRSLEGEA AALRQQQAGR SAMGELYERE VREMRGAVLR LGAARGQLRL EQEHLLEDIA HVRQRLDDEA RQREEAEAAA RALARFAQEA EAARV (1-214 aa encoded by BC014185)

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcription factor ETS proto-oncogene 1 contributes to neuropathic pain by regulating histone deacetylase 1 in primary afferent neurons.

Molecular pain

Zheng HL,Sun SY,Jin T,Zhang M,Zeng Y,Liu Q,Yang K,Wei R,Pan Z,Lin F

Sun Feb 12 00:00:00 UTC 2023

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model.

Nature communications

Hu M,Li T,Ma X,Liu S,Li C,Huang Z,Lin Y,Wu R,Wang S,Lu D,Lu T,Men X,Shen S,Huang H,Liu Y,Song K,Jian B,Jiang Y,Qiu W,Liu Q,Lu Z,Cai W

Tue Jul 04 00:00:00 UTC 2023

Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration.

Bioactive materials

Sun Y,Zhang H,Zhang Y,Liu Z,He D,Xu W,Li S,Zhang C,Zhang Z

Sun Oct 01 00:00:00 UTC 2023

Optogenetic Stimulation of mPFC Alleviates White Matter Injury-Related Cognitive Decline after Chronic Ischemia through Adaptive Myelination.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Deng S,Shu S,Zhai L,Xia S,Cao X,Li H,Bao X,Liu P,Xu Y

Wed Feb 01 00:00:00 UTC 2023

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.

ACS nano

Shen S,Cheng X,Zhou L,Zhao Y,Wang H,Zhang J,Sun X,Wang Y,Shu Y,Xu Y,Tao Y,Li M,Lu Z,Cai W,Nie G,Qiu W

Tue Nov 22 00:00:00 UTC 2022

Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.

Journal of neuroinflammation

Liu Q,Zhou S,Wang X,Gu C,Guo Q,Li X,Zhang C,Zhang N,Zhang L,Huang F

Mon Jun 20 00:00:00 UTC 2022

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.

Biomaterials

Dong X,Wu P,Yan L,Liu K,Wei W,Cheng Q,Liang X,Chen Y,Dai H

Sat Jan 01 00:00:00 UTC 2022

Ursolic Acid Ameliorates Spinal Cord Injury in Mice by Regulating Gut Microbiota and Metabolic Changes.

Frontiers in cellular neuroscience

Rong ZJ,Cai HH,Wang H,Liu GH,Zhang ZW,Chen M,Huang YL

Tue May 24 00:00:00 UTC 2022

Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.

Journal of chemical neuroanatomy

Zhou H,Yin C,Zhang Z,Tang H,Shen W,Zha X,Gao M,Sun J,Xu X,Chen Q

Tue Sep 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC

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Gene Aliases: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

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UniProt ID: (Human) Q9UJS7, (Mouse) P19246

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Entrez Gene ID: (Human) 4744, (Rat) 24587, (Mouse) 380684

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Function(s)

structural molecule activity structural constituent of cytoskeleton microtubule binding toxic substance binding kinesin binding protein kinase binding protein binding, bridging dynein binding protein heterodimerization activity nutrient reservoir activity

Process(es)

microtubule cytoskeleton organization ovarian follicle atresia axonogenesis spinal cord development hippocampus development cerebral cortex development cell projection assembly axon regeneration neurofilament bundle assembly cellular response to oxidative stress response to cocaine peripheral nervous system neuron axonogenesis axon development cellular response to estradiol stimulus regulation of organelle transport along microtubule response to sodium arsenite response to acrylamide brain development embryo development neuron projection development intermediate filament cytoskeleton organization intermediate filament bundle assembly neurofilament cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NF-H Monoclonal Antibody (1A3C7)

NF-H Antibody (60331-1-IG) in ICC/IF

Product Details

60331-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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