Proteintech

NF-M Polyclonal Antibody

NF-M Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

NF-M Antibody (25805-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NF-M Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

25805-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NF-M Fusion Protein Ag22709 (1-97 aa encoded by BC002421)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes endogenous NF-M protein.

Immunogen sequence: MSYTLDSLG NPSAYRRVTE TRSSFSRVSG SPSSGFRSQS WSRGSPSTVS SSYKRSMLAP RLAYSSAMLS SAESSLDFSQ SSSLLNGGSG PGGDYKLS (1-97 aa encoded by BC002421)

Target Information

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury.

Bioactive materials

Yang Q,Su S,Liu S,Yang S,Xu J,Zhong Y,Yang Y,Tian L,Tan Z,Wang J,Yu Z,Shi Z,Liang F

Tue Aug 01 00:00:00 UTC 2023

Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury.

Bioactive materials

Yang Q,Su S,Liu S,Yang S,Xu J,Zhong Y,Yang Y,Tian L,Tan Z,Wang J,Yu Z,Shi Z,Liang F

Tue Aug 01 00:00:00 UTC 2023

Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-κB pathway in LPC-induced demyelination.

Journal of neuroinflammation

Wang Y,Sadike D,Huang B,Li P,Wu Q,Jiang N,Fang Y,Song G,Xu L,Wang W,Xie M

Sat Feb 18 00:00:00 UTC 2023

Time-dependent alterations in the rat nigrostriatal system after intrastriatal injection of fibrils formed by α-Syn and tau fragments.

Frontiers in aging neuroscience

Yang X,Wang J,Zeng W,Zhang X,Yang X,Xu Y,Xu Y,Cao X

Wed Dec 21 00:00:00 UTC 2022

3D-printed collagen/silk fibroin/secretome derived from bFGF-pretreated HUCMSCs scaffolds enhanced therapeutic ability in canines traumatic brain injury model.

Frontiers in bioengineering and biotechnology

Liu X,Zhang G,Wei P,Hao L,Zhong L,Zhong K,Liu C,Liu P,Feng Q,Wang S,Zhang J,Tian R,Zhou L

Tue Sep 13 00:00:00 UTC 2022

Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.

Neuroscience letters

Zhang A,Bai Z,Yi W,Hu Z,Hao J

Tue Sep 14 00:00:00 UTC 2021

Implantation of regenerative complexes in traumatic brain injury canine models enhances the reconstruction of neural networks and motor function recovery.

Theranostics

Jiang J,Dai C,Liu X,Dai L,Li R,Ma K,Xu H,Zhao F,Zhang Z,He T,Niu X,Chen X,Zhang S

Wed Aug 04 00:00:00 UTC 2021

Vagus Nerve Stimulation in Early Stage of Acute Myocardial Infarction Prevent Ventricular Arrhythmias and Cardiac Remodeling.

Frontiers in cardiovascular medicine

Zhao S,Dai Y,Ning X,Tang M,Zhao Y,Li Z,Zhang S

Fri May 14 00:00:00 UTC 2021

Lidocaine prevents breast cancer growth by targeting neuronatin to inhibit nerve fibers formation.

The Journal of toxicological sciences

Li B,Xu H,He C,Zou W,Tu Y

Fri Nov 19 00:00:00 UTC 2021

Study of the protective effect on damaged intestinal epithelial cells of rat multilineage-differentiating stress-enduring (Muse) cells.

Cell biology international

Sun D,Yang L,Cao H,Shen ZY,Song HL

Sat Feb 01 00:00:00 UTC 2020

3D printing collagen/heparin sulfate scaffolds boost neural network reconstruction and motor function recovery after traumatic brain injury in canine.

Biomaterials science

Jiang J,Liu X,Chen H,Dai C,Niu X,Dai L,Chen X,Zhang S

Sat Nov 21 00:00:00 UTC 2020

RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.

Molecular autism

Pacheco NL,Heaven MR,Holt LM,Crossman DK,Boggio KJ,Shaffer SA,Flint DL,Olsen ML

Mon Feb 05 00:00:00 UTC 2018

RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.

Molecular autism

Pacheco NL,Heaven MR,Holt LM,Crossman DK,Boggio KJ,Shaffer SA,Flint DL,Olsen ML

Mon Feb 05 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: 160 kDa neurofilament protein; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NMC

View more View less

Gene Aliases: NEF3; NEFM; NF-M; NF160; NF165; NFM

View more View less

UniProt ID: (Human) P07197, (Mouse) P08553, (Rat) P12839

View more View less

Entrez Gene ID: (Human) 4741, (Mouse) 18040, (Rat) 24588

View more View less

Function(s)

structural constituent of cytoskeleton protein binding microtubule binding receptor binding structural molecule activity toxic substance binding protein heterodimerization activity

Process(es)

neurofilament bundle assembly axon development mitophagy in response to mitochondrial depolarization microtubule cytoskeleton organization axo-dendritic transport regulation of axon diameter intermediate filament cytoskeleton organization intermediate filament polymerization or depolymerization intermediate filament bundle assembly neurofilament cytoskeleton organization spinal cord development axon regeneration cellular response to oxidative stress cellular response to estradiol stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NF-M Polyclonal Antibody

NF-M Antibody (25805-1-AP) in IHC (P)

Product Details

25805-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!