Invitrogen

NFATC2 Monoclonal Antibody (25A10.D6.D2)

NFATC2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 25

NFATC2 Antibody (MA1-025) in ICC/IF

Immunofluorescence analysis of NFATC2 Monoclonal Antibody (25A10.D6.D2) was performed using 70% confluent log phase HeLa and IMR32 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with NFATC2 Monoclonal Antibody (Product # MA1-025) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary An... View More

NFATC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFATC2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

MA1-025

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µL

View 4 publications 4 publications

Gel Shift (GS)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

25A10.D6.D2

Immunogen

Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2152763

Product Specific Information

MA1-025 detects NFATc2 (NFAT1) from mouse, rat and human tissues. This antibody does not cross react with NFAT2 (NFATc, NFATc1).

MA1-025 has been successfully used in Western blot, ChIP, immunocytochemistry, immunoprecipitation, immunofluorescence, immunohistochemistry (frozen & paraffin), and gel shift procedures. By Western blot, this antibody detects an ~140 kDa protein representing phosphorylated NFAT1 in resting immune cells, and an ~120 kDa protein in stimulated cells that represents fully-dephosphorylated NFAT1.

The MA1-025 immunogen is a synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFAT1-A, B and C isoforms. The sequence of this N-terminal peptide is ~70% homologous with human NFAT1.

Antibodies to this protein were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell-based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent DyLight conjugated secondary antibody, may have to be performed for your high content assay.

MA1-025 can be used with blocking peptide PEP-062.

Target Information

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

X-ray irradiation triggers immune response in human T-lymphocytes via store-operated Ca2+ entry and NFAT activation.

The Journal of general physiology

Tandl D,Sponagel T,Alansary D,Fuck S,Smit T,Hehlgans S,Jakob B,Fournier C,Niemeyer BA,Rödel F,Roth B,Moroni A,Thiel G

Published figure using NFATC2 monoclonal antibody (Product # MA1-025) in Immunocytochemistry

Mon May 02 00:00:00 UTC 2022

Forced expression of the non-coding RNA miR-17∼92 restores activation and function in CD28-deficient CD4⁺ T cells.

iScience

Dölz M,Hasiuk M,Gagnon JD,Kornete M,Marone R,Bantug G,Kageyama R,Hess C,Ansel KM,Seyres D,Roux J,Jeker LT

MA1-025 was used in Flow Cytometry, ChIP Assay, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to propose that miR-17∼92 constitutes a central mediator for T cell activation, integrating signals by the TCR and CD28 costimulation by dampening multiple brakes that prevent T cell activation.

Fri Nov 18 00:00:00 UTC 2022

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Immunity

Xian H,Liu Y,Rundberg Nilsson A,Gatchalian R,Crother TR,Tourtellotte WG,Zhang Y,Aleman-Muench GR,Lewis G,Chen W,Kang S,Luevanos M,Trudler D,Lipton SA,Soroosh P,Teijaro J,de la Torre JC,Arditi M,Karin M,Sanchez-Lopez E

MA1-025 was used in ChIP assay to conclude that inhibition of ATP and mtDNA synthesis is sufficient for ARDS amelioration.

Tue Jul 13 00:00:00 UTC 2021

DYRK1A activates NFATC1 to increase glioblastoma migration.

Cancer medicine

Liu H,Sun Q,Chen S,Chen L,Jia W,Zhao J,Sun X

MA1-025 was used in Western Blotting to propose that the recovery of NFATC1 stability is a key oncogenic event in a large proportion of gliomas, and pharmacological inhibition of DYRK1A by polypeptides could represent a promising therapeutic intervention for GBM.

Wed Sep 01 00:00:00 UTC 2021

Nitric oxide attenuates microglia proliferation by sequentially facilitating calcium influx through TRPV2 channels, activating NFATC2, and increasing p21 transcription.

Cell cycle (Georgetown, Tex.)

Maksoud MJE,Tellios V,Lu WY

MA1-025 was used in Immunocytochemistry to indicate that iNOS/NO signaling inhibits microglial proliferation through TRPV2-mediated calcium influx, nuclear translocation of the transcription factor NFATC2, and p21 expression.

Mon Feb 01 00:00:00 UTC 2021

The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression.

Immunity

Tyagi AM,Yu M,Darby TM,Vaccaro C,Li JY,Owens JA,Hsu E,Adams J,Weitzmann MN,Jones RM,Pacifici R

MA1-025 was used in Western Blotting to show butyrate concentrations regulate bone anabolism via Treg cell-mediated regulation of CD8+ T cell Wnt10b production.

Tue Dec 18 00:00:00 UTC 2018

The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression.

Immunity

Tyagi AM,Yu M,Darby TM,Vaccaro C,Li JY,Owens JA,Hsu E,Adams J,Weitzmann MN,Jones RM,Pacifici R

MA1-025 was used in Western Blotting to show butyrate concentrations regulate bone anabolism via Treg cell-mediated regulation of CD8+ T cell Wnt10b production.

Tue Dec 18 00:00:00 UTC 2018

Unique properties of TCR-activated p38 are necessary for NFAT-dependent T-cell activation.

PLoS biology

Alam MS,Gaida MM,Debnath S,Tagad HD,Miller Jenkins LM,Appella E,Rahman MJ,Ashwell JD

Published figure using NFATC2 monoclonal antibody (Product # MA1-025) in Immunofluorescence

Mon Jan 01 00:00:00 UTC 2018

Unique properties of TCR-activated p38 are necessary for NFAT-dependent T-cell activation.

PLoS biology

Alam MS,Gaida MM,Debnath S,Tagad HD,Miller Jenkins LM,Appella E,Rahman MJ,Ashwell JD

Published figure using NFATC2 monoclonal antibody (Product # MA1-025) in Immunofluorescence

Mon Jan 01 00:00:00 UTC 2018

SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation.

eLife

Sarikhani M,Mishra S,Maity S,Kotyada C,Wolfgeher D,Gupta MP,Singh M,Sundaresan NR

Published figure using NFATC2 monoclonal antibody (Product # MA1-025) in Western Blot

Mon Mar 05 00:00:00 UTC 2018

Autocrine lysophosphatidic acid signaling activates β-catenin and promotes lung allograft fibrosis.

The Journal of clinical investigation

Cao P,Aoki Y,Badri L,Walker NM,Manning CM,Lagstein A,Fearon ER,Lama VN

Published figure using NFATC2 monoclonal antibody (Product # MA1-025) in Western Blot

Mon Apr 03 00:00:00 UTC 2017

Deficiency of N-myristoylation reveals calcineurin activity as regulator of IFN-γ-producing γδ T cells.

Journal of leukocyte biology

Rampoldi F,Brunk F,Bonrouhi M,Federico G,Krunic D,Porubsky S,Gröne HJ,Popovic ZV

MA1025 was used in immunocytochemistry to evaluate the potential role of the attachment of myristic acid to the N-terminal glycine of proteins on the activation of gamma delta T cells

Sat Apr 01 00:00:00 UTC 2017

Dual-specificity tyrosine-phosphorylation regulated kinase 1A Gene Transcription is regulated by Myocyte Enhancer Factor 2D.

Scientific reports

Wang P,Wang L,Chen L,Sun X

MA1-025 was used in Chromatin immunoprecipitation to demonstrate that MEF2D could upregulate DYRK1A gene expression through specific activation of DYRK1A isoform 5 gene transcription.

Thu Aug 03 00:00:00 UTC 2017

Toll-Like Receptors Promote Mitochondrial Translocation of Nuclear Transcription Factor Nuclear Factor of Activated T-Cells in Prolonged Microglial Activation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ma B,Yu J,Xie C,Sun L,Lin S,Ding J,Luo J,Cai H

MA1-025 was used in Western Blotting to reveal a previously undescribed mitochondrial translocation of NFAT in microglia responding to extended activation of Toll-like receptor-mediated signalling transduction pathways.

Wed Jul 29 00:00:00 UTC 2015

Toll-Like Receptors Promote Mitochondrial Translocation of Nuclear Transcription Factor Nuclear Factor of Activated T-Cells in Prolonged Microglial Activation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ma B,Yu J,Xie C,Sun L,Lin S,Ding J,Luo J,Cai H

Wed Jul 29 00:00:00 UTC 2015

Nuclear factor of activated T-cells (NFAT)C2 inhibits Notch receptor signaling in osteoblasts.

The Journal of biological chemistry

Zanotti S,Smerdel-Ramoya A,Canalis E

MA1-025 was used in western blot to study the ability of NFATc2 to inhibit osteoblast Notch signaling

Fri Jan 04 00:00:00 UTC 2013

Calcineurin/NFAT signalling inhibits myeloid haematopoiesis.

EMBO molecular medicine

Fric J,Lim CX,Koh EG,Hofmann B,Chen J,Tay HS,Mohammad Isa SA,Mortellaro A,Ruedl C,Ricciardi-Castagnoli P

MA1-025 was used in western blot to study the role of calcineurin/NFAT signaling in inhibiting myeloid haematopoiesis

Sun Apr 01 00:00:00 UTC 2012

Calcineurin/NFAT signalling inhibits myeloid haematopoiesis.

EMBO molecular medicine

Fric J,Lim CX,Koh EG,Hofmann B,Chen J,Tay HS,Mohammad Isa SA,Mortellaro A,Ruedl C,Ricciardi-Castagnoli P

MA1-025 was used in western blot to study the role of calcineurin/NFAT signaling in inhibiting myeloid haematopoiesis

Sun Apr 01 00:00:00 UTC 2012

Cutting edge: Regulatory T cells selectively attenuate, not terminate, T cell signaling by disrupting NF-κB nuclear accumulation in CD4 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Huang YH,Sojka DK,Fowell DJ

MA1-025 was used in flow cytometry to investigate the mechanism of the suppression of CD4 T cell signaling by regulatory T cells

Wed Feb 01 00:00:00 UTC 2012

Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.

Journal of cell science

Gaudineau B,Fougère M,Guaddachi F,Lemoine F,de la Grange P,Jauliac S

MA1-025 was used in ChIP assay to study the regulation of breast cancer invasiveness by NFAT1 and the role played by lipocalin-2, TWEAKR and TWEAK

Mon Oct 01 00:00:00 UTC 2012

Retinoic acid-induced CCR9 expression requires transient TCR stimulation and cooperativity between NFATc2 and the retinoic acid receptor/retinoid X receptor complex.

Journal of immunology (Baltimore, Md. : 1950)

Ohoka Y,Yokota A,Takeuchi H,Maeda N,Iwata M

MA1-025 was used in immunoprecipitation to investigate the cooperativity between NFATc2 and the RAR/RXR and its effect on CCR9 expression

Sat Jan 15 00:00:00 UTC 2011

NFATc1 regulation of TRAIL expression in human intestinal cells.

PloS one

Wang Q,Zhou Y,Weiss HL,Chow CW,Evers BM

MA1-025 was used in EMSA to investigate the regulatory effect of NFATc1 on TRAIL in human intestinal cells

Fri Oct 21 00:00:00 UTC 2011

Pivotal Advance: Nonfunctional lung effectors exhibit decreased calcium mobilization associated with reduced expression of ORAI1.

Journal of leukocyte biology

Arimilli S,Sharma SK,Yammani R,Reid SD,Parks GD,Alexander-Miller MA

MA1-025 was used in immunocytochemistry to identify how the function of lung effector cells is inactivated

Tue Jun 01 00:00:00 UTC 2010

FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression.

Journal of immunology (Baltimore, Md. : 1950)

Torgerson TR,Genin A,Chen C,Zhang M,Zhou B,Añover-Sombke S,Frank MB,Dozmorov I,Ocheltree E,Kulmala P,Centola M,Ochs HD,Wells AD,Cron RQ

MA1-025 was used in chromatin immunoprecipitation to study the effect of FOXP3 on NFAT-mediated inflammatory gene expression.

Wed Jul 15 00:00:00 UTC 2009

Hypertrophy and transcriptional regulation induced in myogenic cell line L6-C5 by an increase of extracellular calcium.

Journal of cellular physiology

De Arcangelis V,Coletti D,Canato M,Molinaro M,Adamo S,Reggiani C,Naro F

MA1-025 was used in western blot to investigate the effect of calcium concentration on cell growth and gene expresion in myogenic cell line L6-C5

Tue Mar 01 00:00:00 UTC 2005

Calcium-dependent activation of interleukin-21 gene expression in T cells.

The Journal of biological chemistry

Kim HP,Korn LL,Gamero AM,Leonard WJ

MA1-025 was used in EMSA to demonstrate the induction of the IL-21 in preactivated T lymphocytes by a calcium signal.

Fri Jul 01 00:00:00 UTC 2005

TRPC3 channels confer cellular memory of recent neuromuscular activity.

Proceedings of the National Academy of Sciences of the United States of America

Rosenberg P,Hawkins A,Stiber J,Shelton JM,Hutcheson K,Bassel-Duby R,Shin DM,Yan Z,Williams RS

Tue Jun 22 00:00:00 UTC 2004

A T cell-specific enhancer of the human CD40 ligand gene.

The Journal of biological chemistry

Schubert LA,Cron RQ,Cleary AM,Brunner M,Song A,Lu LS,Jullien P,Krensky AM,Lewis DB

MA1-025 was used in EMSA assay to study the transcriptional regulation of human CD40 ligand gene

Fri Mar 01 00:00:00 UTC 2002

Regulation of the murine Nfatc1 gene by NFATc2.

The Journal of biological chemistry

Zhou B,Cron RQ,Wu B,Genin A,Wang Z,Liu S,Robson P,Baldwin HS

MA1-025 was used in EMSA to investigate the regulatory effect of NFATc2 on the murine Nfatc1 gene.

Fri Mar 22 00:00:00 UTC 2002

Double-stranded RNA regulates IL-4 expression.

Journal of immunology (Baltimore, Md. : 1950)

Kehoe KE,Brown MA,Imani F

MA1-025 was used in EMSA to investigate the role of the low concentrations of dsRNA towards the IL-4 expression.

Sat Sep 01 00:00:00 UTC 2001

Identification and characterization of a novel nuclear factor of activated T-cells-1 isoform expressed in mouse brain.

The Journal of biological chemistry

Plyte S,Boncristiano M,Fattori E,Galvagni F,Paccani SR,Majolini MB,Oliviero S,Ciliberto G,Telford JL,Baldari CT

MA1-025 was used in western blot to show that NFAT1-D is expressed in the mouse brain

Fri Apr 27 00:00:00 UTC 2001

The calcineurin-NFAT pathway and muscle fiber-type gene expression.

American journal of physiology. Cell physiology

Swoap SJ,Hunter RB,Stevenson EJ,Felton HM,Kansagra NV,Lang JM,Esser KA,Kandarian SC

MA1-025 was used in western blot to investigate the role of calcineurin-NFAT pathway in the regulation of muscle fiber-type gene expression

Sun Oct 01 00:00:00 UTC 2000

Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway.

Science (New York, N.Y.)

Chow CW,Rincón M,Cavanagh J,Dickens M,Davis RJ

MA1-025 was used in immunohistochemistry to demonstrate that nuclear accumulation of NFAT4 is regulated by JNK

Fri Nov 28 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: IB-IXL; KIAA0611; NF-ATc2; NF-ATp; NFAT pre-existing s; NFAT pre-existing subunit; NFAT transcription complex, preexisting component; Nuclear factor of activated T-cells, cytoplasmic 2; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; nuclear factor of activated T-cells, preexisting component; OTTHUMP00000031291; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1

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Gene Aliases: AI607462; NF-ATc2; NF-ATp; NFAT1; NFAT1-D; NFATC2; NFATP

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UniProt ID: (Human) Q13469, (Mouse) Q60591

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Entrez Gene ID: (Human) 4773, (Rat) 311658, (Mouse) 18019

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding molecular_function sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter cytokine production transcription from RNA polymerase II promoter cellular response to DNA damage stimulus biological_process positive regulation of gene expression myotube cell development cell migration positive regulation of B cell proliferation calcineurin-NFAT signaling cascade response to drug positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter B cell receptor signaling pathway positive regulation of myoblast fusion regulation of transcription, DNA-templated Fc-epsilon receptor signaling pathway transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFATC2 Monoclonal Antibody (25A10.D6.D2)

NFATC2 Antibody (MA1-025) in ICC/IF

Product Details

MA1-025

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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