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Invitrogen
NFATC3 Polyclonal Antibody
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NFATC3 Antibody (PA5-79734) in WB
Western blot analysis of NFATC3 in Lane 1: human 22RV1 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with NFATC3 polyclonal antibody (Product # PA5-79734) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP second... View More
Product Details
PA5-79734
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human NFAT4 recombinant protein (Position: K630-L712).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746849
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 22RV1 whole cell. Flow: A431 cell, U20S cell, K562 cell, SiHa cell.
Target Information
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: NF-AT4; NF-ATc3; NFAC3; NFATx; NFC3; nuclear factor of activated T-cells c3 isoform IE-Xa; Nuclear factor of activated T-cells, cytoplasmic 3; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4; T-cell transcription factor NFAT4
Gene Aliases: C80703; D8Ertd281e; NFAT4; NFATC3; NFATX
UniProt ID: (Human) Q12968
Entrez Gene ID: (Human) 4775, (Rat) 361400, (Mouse) 18021
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor binding
sequence-specific DNA binding
patterning of blood vessels
response to hypoxia
blood vessel remodeling
regulation of transcription, DNA-templated
heart development
cardiac muscle hypertrophy in response to stress
myotube differentiation
myotube cell development
T cell differentiation
calcineurin-NFAT signaling cascade
cellular respiration
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
thymus development
skeletal muscle fiber development
smooth muscle cell differentiation
muscle cell development
cellular response to calcium ion
cellular response to lithium ion
regulation of store-operated calcium entry
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
inflammatory response
Fc-epsilon receptor signaling pathway
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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