Invitrogen

NFATC4 Polyclonal Antibody

NFATC4 Antibody in Immunocytochemistry (ICC/IF)

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NFATC4 Antibody (PA1-021) in ICC/IF

Immunofluorescent analysis of NFAT3 using anti-NFAT3 polyclonal antibody (Product # PA1-021) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing NFAT3 (Product # PA1-021), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goa... View More

NFATC4 Antibody in Immunoprecipitation (IP)

NFATC4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

NFATC4 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-021

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:3000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

3 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C R(887) D L S G F P A P P G E E P P A(902) of human NFAT3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2267268

Product Specific Information

PA1-021 detects nuclear factor of activated T-cells 3 (NFAT3) from human cells.

PA1-021 has been successfully used in Western blot immunohistochemistry, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing NFAT3 in HEK293 cells transfected with the human NFAT3 gene. Immunohistochemical staining of NFAT3 in HEK293 cells transfected with the human NFAT3 gene results in cytoplasmic staining.

The PA1-021 immunogen is a synthetic peptide corresponding to residues C R(887) D L S G F P A P P G E E P P A(902) of human NFAT3. This sequence is 94% conserved with mouse NFAT3. The PA1-021 immunizing peptide (Cat. # PEP-122) is available for use in neutralization and control experiments.

Target Information

This protein is an adapter or scaffold protein, which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elucidation of GPR55-Associated Signaling behind THC and LPI Reducing Effects on Ki67-Immunoreactive Nuclei in Patient-Derived Glioblastoma Cells.

Cells

Kolbe MR,Hohmann T,Hohmann U,Maronde E,Golbik R,Prell J,Illert J,Strauss C,Dehghani F

PA1-021 was used in Immunocytochemistry to investigate intracellular mechanisms leading to an altered number of Ki67+ nuclei after stimulation of GPR55 by LPI and THC.

Fri Nov 17 00:00:00 UTC 2023

Inhibition of Syk promotes chemical reprogramming of fibroblasts via metabolic rewiring and H₂ S production.

The EMBO journal

Wang W,Ren S,Lu Y,Chen X,Qu J,Ma X,Deng Q,Hu Z,Jin Y,Zhou Z,Ge W,Zhu Y,Yang N,Li Q,Pu J,Chen G,Ye C,Wang H,Zhao X,Liu Z,Zhu S

PA1-021 was used in Immunocytochemistry-immunoflourescence to show that inhibition of spleen tyrosine kinase by R406 significantly promotes mouse chemical reprogramming.

Tue Jun 01 00:00:00 UTC 2021

Transcriptional Profiling of Hypoxic Neural Stem Cells Identifies Calcineurin-NFATc4 Signaling as a Major Regulator of Neural Stem Cell Biology.

Stem cell reports

Moreno M,Fernández V,Monllau JM,Borrell V,Lerin C,de la Iglesia N

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Tue Aug 11 00:00:00 UTC 2015

Transcriptional Profiling of Hypoxic Neural Stem Cells Identifies Calcineurin-NFATc4 Signaling as a Major Regulator of Neural Stem Cell Biology.

Stem cell reports

Moreno M,Fernández V,Monllau JM,Borrell V,Lerin C,de la Iglesia N

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Tue Aug 11 00:00:00 UTC 2015

Nuclear factor of activated T cells (NFAT) signaling regulates PTEN expression and intestinal cell differentiation.

Molecular biology of the cell

Wang Q,Zhou Y,Jackson LN,Johnson SM,Chow CW,Evers BM

PA1-021 was used in immunohistochemistry and western blot to confirm the role of NFATc1 in PTEN expression and intestinal cell differentiation

Tue Feb 01 00:00:00 UTC 2011

Nuclear factor of activated T cells (NFAT) signaling regulates PTEN expression and intestinal cell differentiation.

Molecular biology of the cell

Wang Q,Zhou Y,Jackson LN,Johnson SM,Chow CW,Evers BM

PA1-021 was used in immunohistochemistry and western blot to confirm the role of NFATc1 in PTEN expression and intestinal cell differentiation

Tue Feb 01 00:00:00 UTC 2011

NFAT-3 is a transcriptional repressor of the growth-associated protein 43 during neuronal maturation.

The Journal of biological chemistry

Nguyen T,Lindner R,Tedeschi A,Forsberg K,Green A,Wuttke A,Gaub P,Di Giovanni S

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Fri Jul 10 00:00:00 UTC 2009

NFAT-3 is a transcriptional repressor of the growth-associated protein 43 during neuronal maturation.

The Journal of biological chemistry

Nguyen T,Lindner R,Tedeschi A,Forsberg K,Green A,Wuttke A,Gaub P,Di Giovanni S

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Fri Jul 10 00:00:00 UTC 2009

Phosphorylation of NFATc4 by p38 mitogen-activated protein kinases.

Molecular and cellular biology

Yang TT,Xiong Q,Enslen H,Davis RJ,Chow CW

PA1-021 was used in western blot to study the function of nuclear factor of activated T cells (NFAT) in PPARgamma gene expression and adipocyte differentiation.

Sat Jun 01 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: NF-AT3; NF-ATc4; Nuclear factor of activated T-cells, cytoplasmic 4; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4; T-cell transcription factor NFAT3

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Gene Aliases: NF-AT3; NF-ATC4; NFAT3; NFATC4

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UniProt ID: (Human) Q14934

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Entrez Gene ID: (Human) 4776

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription coactivator activity protein binding transcription factor binding peroxisome proliferator activated receptor binding

Process(es)

patterning of blood vessels transcription from RNA polymerase II promoter inflammatory response heart development intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of Wnt signaling pathway negative regulation of protein binding positive regulation of tumor necrosis factor production calcineurin-NFAT signaling cascade cellular response to UV negative regulation of chromatin binding cellular respiration positive regulation of transcription from RNA polymerase II promoter regulation of synaptic plasticity negative regulation of dendrite morphogenesis smooth muscle cell differentiation muscle cell development cellular response to lithium ion negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of synapse maturation positive regulation of apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFATC4 Polyclonal Antibody

NFATC4 Antibody (PA1-021) in ICC/IF

Product Details

PA1-021

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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