Proteintech

NFE2 Polyclonal Antibody

NFE2 Antibody in Immunocytochemistry (ICC/IF)

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NFE2 Antibody (11089-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using NFE2 antibody 11089-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

NFE2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFE2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11089-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NFE2 Fusion Protein Ag1570 (1-373 aa encoded by BC005044)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin.

Immunogen sequence: MSPCPPQQS RNRVIQLSTS ELGEMELTWQ EIMSITELQG LNAPSEPSFE PQAPAPYLGP PPPTTYCPCS IHPDSGFPLP PPPYELPAST SHVPDPPYSY GNMAIPVSKP LSLSGLLSEP LQDPLALLDI GLPAGPPKPQ EDPESDSGLS LNYSDAESLE LEGTEAGRRR SEYVEMYPVE YPYSLMPNSL AHSNYTLPAA ETPLALEPSS GPVRAKPTAR GEAGSRDERR ALAMKIPFPT DKIVNLPVDD FNELLARYPL TESQLALVRD IRRRGKNKVA AQNCRKRKLE TIVQLERELE RLTNERERLL RARGEADRTL EVMRQQLTEL YRDIFQHLRD ESGNSYSPEE YALQQAADGT IFLVPRGTKM EATD (1-373 aa encoded by BC005044)

Target Information

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Succinylation of CTBP1 mediated by KAT2A suppresses its inhibitory activity on the transcription of CDH1 to promote the progression of prostate cancer.

Biochemical and biophysical research communications

Zhou J,Yan X,Liu Y,Yang J

Sun Apr 02 00:00:00 UTC 2023

Xanthotoxin, a novel inducer of platelet formation, promotes thrombocytopoiesis via IL-1R1 and MEK/ERK signaling.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Lai J,Li Y,Ran M,Huang Q,Huang F,Zhu L,Wu Y,Zou W,Xie X,Tang Y,Yang F,Wu A,Ge G,Wu J

Sat Jul 01 00:00:00 UTC 2023

Targeting TLR2/Rac1/cdc42/JNK Pathway to Reveal That Ruxolitinib Promotes Thrombocytopoiesis.

International journal of molecular sciences

Yang S,Tang X,Wang L,Ni C,Wu Y,Zhou L,Zeng Y,Zhao C,Wu A,Wang Q,Xu X,Wang Y,Chen R,Zhang X,Zou L,Huang X,Wu J

Sat Dec 17 00:00:00 UTC 2022

Targeting TLR2/Rac1/cdc42/JNK Pathway to Reveal That Ruxolitinib Promotes Thrombocytopoiesis.

International journal of molecular sciences

Yang S,Tang X,Wang L,Ni C,Wu Y,Zhou L,Zeng Y,Zhao C,Wu A,Wang Q,Xu X,Wang Y,Chen R,Zhang X,Zou L,Huang X,Wu J

Sat Dec 17 00:00:00 UTC 2022

Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages.

BMC genomics

Arnan C,Ullrich S,Pulido-Quetglas C,Nurtdinov R,Esteban A,Blanco-Fernandez J,Aparicio-Prat E,Johnson R,Pérez-Lluch S,Guigó R

Thu May 26 00:00:00 UTC 2022

Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Lu Z,Huang L,Li Y,Xu Y,Zhang R,Zhou Q,Sun Q,Lu Y,Chen J,Shen Y,Li J,Zhao B

Sat Jan 01 00:00:00 UTC 2022

ERK and p38 MAPK inhibition controls NF-E2 degradation and profibrotic signaling in renal proximal tubule cells.

Life sciences

Li J,Jin S,Barati MT,Rane S,Lin Q,Tan Y,Cai L,Rane MJ

Wed Dec 15 00:00:00 UTC 2021

Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.

Life sciences

Jin S,Li J,Barati M,Rane S,Lin Q,Tan Y,Zheng Z,Cai L,Rane MJ

Sat Aug 01 00:00:00 UTC 2020

Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.

Life sciences

Jin S,Li J,Barati M,Rane S,Lin Q,Tan Y,Zheng Z,Cai L,Rane MJ

Sat Aug 01 00:00:00 UTC 2020

Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.

Life sciences

Jin S,Li J,Barati M,Rane S,Lin Q,Tan Y,Zheng Z,Cai L,Rane MJ

Sat Aug 01 00:00:00 UTC 2020

Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.

Life sciences

Jin S,Li J,Barati M,Rane S,Lin Q,Tan Y,Zheng Z,Cai L,Rane MJ

Sat Aug 01 00:00:00 UTC 2020

Heme levels are increased in human failing hearts.

Journal of the American College of Cardiology

Khechaduri A,Bayeva M,Chang HC,Ardehali H

Tue May 07 00:00:00 UTC 2013

Heme levels are increased in human failing hearts.

Journal of the American College of Cardiology

Khechaduri A,Bayeva M,Chang HC,Ardehali H

Tue May 07 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Leucine zipper protein NF-E2; nuclear factor (erythroid-derived 2), 45kD; nuclear factor (erythroid-derived 2), 45kDa; Nuclear factor, erythroid-derived 2 45 kDa subunit; p45 NF-E2; Transcription factor NF-E2 45 kDa subunit

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Gene Aliases: NF-E2; NFE2; p45

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UniProt ID: (Human) Q16621

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Entrez Gene ID: (Human) 4778

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Function(s)

transcription regulatory region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding sequence-specific DNA binding protein N-terminus binding WW domain binding

Process(es)

nucleosome disassembly transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development blood coagulation hemostasis blood circulation positive regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFE2 Polyclonal Antibody

NFE2 Antibody (11089-1-AP) in ICC/IF

Product Details

11089-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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