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Invitrogen
NFIC Polyclonal Antibody
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NFIC Antibody (PA5-90204) in WB
Western blot analysis of NFIC in extracts of various cell lines. Samples were incubated with NFIC Polyclonal antibody (Product # PA5-90204) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-90204
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFIC (NP_0055882)
Conjugate
Form
Liquid
Concentration
2.16 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805958
Product Specific Information
Immunogen sequence: MYSSPLCLTQ DEFHPFIEAL LPHVRAFAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE LLGEKPEVKQ KWASRLLAKL RKDIRPECRE DFVLSITGKK APGCVLSNPD QKGKMRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER LVKAAQCGHP VLCVQPHHIG VAVKELDLYL AYFVRERDAE QSGSPRTGMG SDQEDSKPIT LDTTDFQESF; Positive Samples: HeLa, LO2, MCF-7, DU145, NIH/3T3, mouse liver, mouse skeletal muscle, rat liver; Cellular Location: Nucleus
Target Information
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CCAAT-box-binding transcription factor; CTF; KIAA1439; NF-I/A; NF-I/B; NF-I/C; NF-I/X; NF1-A; NF1-B; NF1-C; NF1-X; NFI-A; NFI-B; NFI-C; NFI-X; NFIA; NFIB; NFIX; Nuclear factor 1 C-type; nuclear factor 1-C2; Nuclear factor 1/A; Nuclear factor 1/B; nuclear factor 1/C; Nuclear factor 1/X; Nuclear factor I/A; Nuclear factor I/B; Nuclear factor I/C; nuclear factor I/C (CCAAT-binding transcription factor); Nuclear factor I/X; olfactory epithelium nuclear factor I-C; TGGCA-binding protein
Gene Aliases: 1110019L22Rik; 1500041O16Rik; AA589446; AI746521; CTF; CTF5; NF-I; NF1-C; NFI; NFIC
UniProt ID: (Human) P08651, (Mouse) P70255
Entrez Gene ID: (Human) 4782, (Mouse) 18029, (Rat) 29228
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region DNA binding
DNA binding
double-stranded DNA binding
protein binding
negative regulation of transcription from RNA polymerase II promoter
DNA replication
transcription from RNA polymerase II promoter
odontogenesis of dentin-containing tooth
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-templated
sensory perception of smell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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