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NFIL3 Polyclonal Antibody

NFIL3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

NFIL3 Antibody (11773-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 11773-1-AP (NFIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NFIL3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11773-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

View 3 publications 3 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NFIL3 Fusion Protein Ag2380 (113-462 aa encoded by BC008197)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ATLKAELLS LKLKFGLISS TAYAQEIQKL SNSTAVYFQD YQTSKSNVSS FVDEHEPSMV SSSCISVIKH SPQSSLSDVS EVSSVEHTQE SSVQGSCRSP ENKFQIIKQE PMELESYTRE PRDDRGSYTA SIYQNYMGNS FSGYSHSPPL LQVNRSSSNS PRTSETDDGV VGKSSDGEDE QQVPKGPIHS PVELKHVHAT VVKVPEVNSS ALPHKLRIKA KAMQIKVEAF DNEFEATQKL SSPIDMTSKR HFELEKHSAP SMVHSSLTPF SVQVTNIQDW SLKSEHWHQK ELSGKTQNSF KTGVVEMKDS GYKVSDPENL YLKQGIANLS AEVVSLKRLI ATQPISASDS G (113-462 aa encoded by BC008197)

Target Information

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription.

Journal of experimental & clinical cancer research : CR

Yang W,Li J,Zhang M,Yu H,Zhuang Y,Zhao L,Ren L,Gong J,Bi H,Zeng L,Xue Y,Yang J,Zhao Y,Wang S,Gao S,Fu Z,Li D,Zhang J,Wang T,Shan M,Tang B,Li X

Fri Feb 18 00:00:00 UTC 2022

Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription.

Journal of experimental & clinical cancer research : CR

Yang W,Li J,Zhang M,Yu H,Zhuang Y,Zhao L,Ren L,Gong J,Bi H,Zeng L,Xue Y,Yang J,Zhao Y,Wang S,Gao S,Fu Z,Li D,Zhang J,Wang T,Shan M,Tang B,Li X

Fri Feb 18 00:00:00 UTC 2022

Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription.

Journal of experimental & clinical cancer research : CR

Yang W,Li J,Zhang M,Yu H,Zhuang Y,Zhao L,Ren L,Gong J,Bi H,Zeng L,Xue Y,Yang J,Zhao Y,Wang S,Gao S,Fu Z,Li D,Zhang J,Wang T,Shan M,Tang B,Li X

Fri Feb 18 00:00:00 UTC 2022

Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice.

Biochemical pharmacology

Chen M,Xiao Y,Zhang F,Du J,Zhang L,Li Y,Lu D,Wang Z,Wu B

Tue Nov 01 00:00:00 UTC 2022

NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.

Frontiers in medicine

Tang H,Tan C,Cao X,Liu Y,Zhao H,Liu Y,Zhao Y

Mon Apr 17 00:00:00 UTC 2023

NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.

Frontiers in medicine

Tang H,Tan C,Cao X,Liu Y,Zhao H,Liu Y,Zhao Y

Mon Apr 17 00:00:00 UTC 2023

Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.

Xenobiotica; the fate of foreign compounds in biological systems

Chen M,Zhou C,Zhang T,Wu B

Tue Sep 01 00:00:00 UTC 2020

The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.

Drug metabolism and disposition: the biological fate of chemicals

Chen M,Guan B,Xu H,Yu F,Zhang T,Wu B

Fri Nov 01 00:00:00 UTC 2019

The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.

Drug metabolism and disposition: the biological fate of chemicals

Chen M,Guan B,Xu H,Yu F,Zhang T,Wu B

Fri Nov 01 00:00:00 UTC 2019

NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression.

BioMed research international

Xu J,Xu G,Zhang T,Chen T,Zhao W,Wang G

Wed May 13 00:00:00 UTC 2020

The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.

Theranostics

Yu F,Zhang T,Zhou C,Xu H,Guo L,Chen M,Wu B

Thu Jun 18 00:00:00 UTC 2020

The transcription factor E4bp4 regulates the expression and activity of Cyp3a11 in mice.

Biochemical pharmacology

Tong Y,Zeng P,Zhang T,Zhao M,Yu P,Wu B

Wed May 01 00:00:00 UTC 2019

Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.

Neuropharmacology

Li C,Zhang J,Xu H,Chang M,Lv C,Xue W,Song Z,Zhang L,Zhang X,Tian X

Fri Jun 01 00:00:00 UTC 2018

Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.

Neuropharmacology

Li C,Zhang J,Xu H,Chang M,Lv C,Xue W,Song Z,Zhang L,Zhang X,Tian X

Fri Jun 01 00:00:00 UTC 2018

Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.

Brain : a journal of neurology

Shiihashi G,Ito D,Yagi T,Nihei Y,Ebine T,Suzuki N

Thu Sep 01 00:00:00 UTC 2016

Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.

Brain : a journal of neurology

Shiihashi G,Ito D,Yagi T,Nihei Y,Ebine T,Suzuki N

Thu Sep 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: E4 promoter binding-protein 4; E4 promoter-binding protein 4; Embryo implantation-related NFIL3/E4BP4-like transcription factor; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NF IL3A; nuclear factor interleukin 3 regulated protein; Nuclear factor interleukin-3-regulated protein; OTTHUMP00000021633; Transcriptional activator NF-IL3A

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Gene Aliases: AV225605; E4BP4; IL3BP1; NF-IL3A; NFIL3; NFIL3A

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UniProt ID: (Human) Q16649, (Mouse) O08750, (Rat) Q6IMZ0

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Entrez Gene ID: (Human) 4783, (Mouse) 18030, (Rat) 114519

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter immune response circadian rhythm positive regulation of gene expression rhythmic process cellular response to interleukin-4

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFIL3 Polyclonal Antibody

NFIL3 Antibody (11773-1-AP) in ICC/IF

Product Details

11773-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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