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NFYB Antibody (PA5-31913) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of NFYB was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Product Details
PA5-31913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549386
Product Specific Information
Recommended positive controls: HeLa, HeLa nuclear extract, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold "handshake" motif in a manner analogous to the histone proteins H2B and H2A, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CAAT box DNA-binding protein subunit B; CAAT-box DNA-binding protein subunit B; CBF-A; CBF-B; CCAAT binding transcription factor of CBF-B/NFY-B; CCAAT-binding transcription factor subunit A; CCAAT-binding transcription factor subunit B; nf-y; NF-YB; nuclear transcription factor - Y beta; Nuclear transcription factor Y subunit B; Nuclear transcription factor Y subunit beta; nuclear transcription factor Y, beta; Transcription factor NF-Y, B subunit
Gene Aliases: AA985999; CBF-A; CBF-B; CBFB; HAP3; NF-YB; Nfya; NFYB
UniProt ID: (Human) P25208, (Mouse) P63139, (Rat) P63140
Entrez Gene ID: (Human) 4801, (Mouse) 18045, (Rat) 25336
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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