Invitrogen

NFkB p50 Recombinant Superclonal™ Antibody (24HCLC)

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2 Published Figures
1 Reference
View all (15) NFkB p50 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

710450

Applications
Tested Dilution
Publications

Western Blot (WB)

2-3 µg/mL
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Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

ChIP assay (ChIP)

1-5 µg x 10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

24HCLC

Immunogen

Peptide corresponding to amino acids 143-156 of human p50
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532720

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor. Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex. The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686C01211; DNA-binding factor KBF1; EBP-1; MGC54151; NF kappaB1; NF KB; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-kappaB p50; NF-kappabeta; NF-kB p50; nuclear factor; nuclear factor kappa b; nuclear factor kappa-B DNA binding subunit; nuclear factor kappaB p50; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105; nuclear factor-kappaB p50; OTTHUMP00000219572; p50 subunit of NF kappaB; p50 subunit of NF-kappaB

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Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kappaB1; NF-KB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; p105; p50; p50/p105

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UniProt ID: (Human) P19838, (Mouse) P25799

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Entrez Gene ID: (Human) 4790, (Mouse) 18033

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Function(s)
regulatory region DNA binding transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding heat shock protein binding identical protein binding protein homodimerization activity actinin binding transcription regulatory region DNA binding protein heterodimerization activity RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding double-stranded DNA binding sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter negative regulation of cytokine production transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter apoptotic process inflammatory response response to oxidative stress signal transduction I-kappaB kinase/NF-kappaB signaling negative regulation of calcidiol 1-monooxygenase activity response to cytokine response to muscle stretch NIK/NF-kappaB signaling negative regulation of apoptotic process negative regulation of interleukin-12 biosynthetic process innate immune response negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter lymph node development negative regulation of inflammatory response cellular response to lipopolysaccharide cellular response to nicotine cellular response to interleukin-6 cellular response to dsRNA cellular response to peptide hormone stimulus positive regulation of canonical Wnt signaling pathway positive regulation of hyaluronan biosynthetic process positive regulation of miRNA metabolic process stimulatory C-type lectin receptor signaling pathway positive regulation of macrophage derived foam cell differentiation positive regulation of lipid storage negative regulation of vitamin D biosynthetic process membrane protein intracellular domain proteolysis negative regulation of cellular protein metabolic process negative regulation of cholesterol transport positive regulation of type I interferon production Fc-epsilon receptor signaling pathway response to copper ion T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade cellular response to mechanical stimulus cellular response to carbohydrate stimulus cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to diterpene cellular response to glucoside cellular response to brain-derived neurotrophic factor stimulus
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