Invitrogen

NFkB p65 Polyclonal Antibody, eBioscience™

NFkB p65 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

NFkB p65 Antibody (14-6731-81) in ICC/IF

Immunofluorescence analysis of Transcription factor p65 was performed using HeLa cells (serum-starved) and HeLa cells serum-starved for 16 hours, followed by 20 ng/mL human TNF alpha treatment for 40 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NFkB p65 Polyclonal Antibody, eBioscience™ (Product # 14-6731-81) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overn... View More

NFkB p65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFkB p65 Antibody in Immunohistochemistry (IHC)

NFkB p65 Antibody in Western Blot, Gel Shift (WB, GS)

Product Image Gallery

Product Details

14-6731-81

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-Dependent

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 0.02% gelatin, BSA

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468255

Product Specific Information

Description: The polyclonal antibody reacts with mouse, rat, and human NFkB p65; the antibody was raised against a peptide mapping to the carboxy terminus of human NFkB p65. Members of the rel/NFkB family of transcription factors are involved in the regulation of cellular responses, such as growth, development, and the inflammatory response. They share a structural motif known as the rel homology region (RHR), the C-terminal one third of which mediates protein dimerization (2, 6, 8). Complexes of p50 (NF-kB1) or p52 (NF-kB2) are generated through the processing of p105 and p100 precursors, respectively. These are usually associated with members of the Rel family (p65, c-Rel, Rel B). The homo- and heterodimer formed through combinations of NFkB/Rel proteins bind distinct kB sites to regulate the transcription of different genes (7, 9). In resting cells, NFkB is retained in the cytoplasm bound to inhibitory proteins of the IkB family. Degradation of IkB proteins occurs with cell activation, via of variety of signals, including inflammatory cytokines and bacterial lipopolysaccharides (LPS) as well as oxidative and fluid mechanical stress. This results in nuclear translocation of NFkB and the transcriptional gene activation of proinflammatory genes (1, 9). It has been suggested that NFkB plays a role in the development of numerous pathological states. Activation of NFkB induces gene programs leading to transcription of factors that promote inflammation, such as leukocyte adhesion molecules, cytokines, and chemokines. It is also thought that there are some substances with possible anti-inflammatory effects that are also NFkB regulated. There is some evidence indicating NFkB as a key factor in the pathophysiology of cardiac ischemia-reperfusion injury as well as the development of insulin dependent Diabetes Mellitus (4, 3).

Applications Reported: Purified anti-mo/hu/rat NFkB p65 poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: Purified anti-mo/hu/rat NFkB p65 poly has been tested by immunoblotting (WB) and immunohistochemistry. (1:1000 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Remifentanil modulates the TLR4‑mediated MMP‑9/TIMP1 balance and NF‑κB/STAT3 signaling in LPS‑induced A549 cells.

Experimental and therapeutic medicine

Chang J,Zhang W

14-6731-81 was used in Western Blotting to show that remifentanil downregulated TLR4 to reduce MMP-9/TIMP1 imbalance to inhibit inflammatory dysfunction in LPS-treated A549 cells, by regulating NF-κB/STAT3 signaling.

Wed Feb 01 00:00:00 UTC 2023

Significance of Plasmodium berghei Amino Acid Transporter 1 in Food Vacuole Functionality and Its Association with Cerebral Pathogenesis.

Microbiology spectrum

Anand A,Chandana M,Ghosh S,Das R,Singh N,Vaishalli PM,Gantasala NP,Padmanaban G,Nagaraj VA

14-6731-81 was used in Western Blotting to show that the deletion of amino acid transporter 1 in Plasmodium berghei leads to swollen food vacuoles with the accumulation of hemoglobin-derived peptides.

Tue Mar 28 00:00:00 UTC 2023

Butorphanol reduces the neuronal inflammatory response and apoptosis via inhibition of p38/JNK/ATF2/p53 signaling.

Experimental and therapeutic medicine

Huang Y,Li S,Chen H,Feng L,Yuan W,Han T

Published figure using NFkB p65 polyclonal antibody (Product # 14-6731-81) in Western Blot

Tue Mar 01 00:00:00 UTC 2022

Malaria parasite heme biosynthesis promotes and griseofulvin protects against cerebral malaria in mice.

Nature communications

Chandana M,Anand A,Ghosh S,Das R,Beura S,Jena S,Suryawanshi AR,Padmanaban G,Nagaraj VA

14-6731-81 was used in Western Blotting to show heme synthesized in asexual stages promotes cerebral pathogenesis by enhancing hemozoin formation.

Tue Jul 12 00:00:00 UTC 2022

Ameliorative Effect of Curcumin Nanoparticles against Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats.

Mediators of inflammation

Hamdalla HM,Ahmed RR,Galaly SR,Naguib IA,Alghamdi BS,Ahmed OM,Farghali A,Abdul-Hamid M

14-6731-81 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot to demonstrate that CUR NPs provides effective protection against MIA-induced OA and knee joint histological deteriorated changes due to its anti-inflammatory, antioxidant, and antiapoptotic properties.

Fri Dec 30 00:00:00 UTC 2022

Anti-obesity and Anti-diabetic Effect of Ursolic Acid against Streptozotocin/High Fat Induced Obese in Diabetic Rats.

Journal of oleo science

Tang S,Fang C,Liu Y,Tang L,Xu Y

14-6731-81 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot to suggest that the anti-obese effect of ursolic acid against high fat diet (HFD) induced obese rats via alteration of insulin and JNK signaling pathway.

Thu Feb 03 00:00:00 UTC 2022

Modulation of allergic inflammation in the lung by a peptide derived from Mycobacteria tuberculosis chaperonin 60.1.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Riffo-Vasquez Y,Kanabar V,Keir SD,E-Lacerda RR,Man F,Jackson DJ,Corrigall V,Coates ARM,Page CP

14-6731 was used in Immunohistochemistry to investigate the effect of a shorter peptide sequence derived from Cpn 60.1, named IRL201104, on allergic lung inflammation induced by ovalbumin (OVA) in mice and by house dust mite (HDM) in guinea pigs, as well as investigating the action of IRL201104 on human cells in vitro.

Wed Apr 01 00:00:00 UTC 2020

DNA methylation is involved in the pathogenesis of osteoarthritis by regulating CtBP expression and CtBP-mediated signaling.

International journal of biological sciences

Sun X,Xiao L,Chen J,Chen X,Chen X,Yao S,Li H,Zhao G,Ma J

14-6731-81 was used in Western Blotting to identify a transactivation mechanism of NLRP3 mediated by CtBPs (C-terminal-binding proteins).

Mon Jun 07 00:00:00 UTC 2021

Palmitic acid induces guanylin gene expression through the Toll-like receptor 4/nuclear factor-κB pathway in rat macrophages.

American journal of physiology. Cell physiology

Akieda-Asai S,Ma H,Date Y

14-6731 was used in Chromatin immunoprecipitation to indicate that saturated fatty acids, such as palmitic acid, activate guanylin gene expression via the nuclear factor-κB pathway in macrophages.

Sun Dec 01 00:00:00 UTC 2019

Inhibitory effects of quail egg on mast cells degranulation by suppressing PAR2-mediated MAPK and NF-kB activation.

Food & nutrition research

Lianto P,Ogutu FO,Zhang Y,He F,Che H

14-6731 was used in Western Blotting to investigate the suppressive role of quail egg in mast cell degranulation and cytokine production of the effect phase response.

Fri Mar 18 00:00:00 UTC 2022

Anti-inhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts.

International journal of molecular medicine

Lee DS,Lee CM,Park SK,Yim MJ,Lee JM,Choi G,Yoo JS,Jung WK,Park S,Seo SK,Park WS,Choi IW

14-6731 was used in Western Blotting to determine the anti-inflammatory effects of Disromium deumbens in nasal polyp-derived fibroblasts and its mechanism of action.

Fri Dec 01 00:00:00 UTC 2017

Free immunoglobulin light chain (FLC) promotes murine colitis and colitis-associated colon carcinogenesis by activating the inflammasome.

Scientific reports

Ma J,Jiang D,Gong X,Shao W,Zhu Z,Xu W,Qiu X

14-6731 was used in Western Blotting to investigate whether free Ig light chain is involved in colitis and colitis-associated colon carcinogenesis.

Wed Jul 12 00:00:00 UTC 2017

Anti-hepatitis B virus (HBV) response of imiquimod based toll like receptor 7 ligand in hbv-positive human hepatocelluar carcinoma cell line.

BMC infectious diseases

Das D,Sengupta I,Sarkar N,Pal A,Saha D,Bandopadhyay M,Das C,Narayan J,Singh SP,Chakrabarti S,Chakravarty R

Published figure using NFkB p65 polyclonal antibody (Product # 14-6731-81) in Immunofluorescence

Sat Jan 14 00:00:00 UTC 2017

Neuroprotective and anti-inflammatory effects of morin in a murine model of Parkinson's disease.

Journal of neuroscience research

Lee KM,Lee Y,Chun HJ,Kim AH,Kim JY,Lee JY,Ishigami A,Lee J

14-6731-81 was used in Immunocytochemistry-immunofluorescence to indicate that morin acts via multiple neuroprotective mechanisms in our mouse model and suggest that morin be viewed as a potential treatment and preventative for PD.

Sat Oct 01 00:00:00 UTC 2016

YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways.

International journal of molecular medicine

Paeng SH,Park WS,Jung WK,Lee DS,Kim GY,Choi YH,Seo SK,Jang WH,Choi JS,Lee YM,Park S,Choi IW

14-6731-81 was used in Western Blot to suggest that this anti-inflammatory activity occurred through ROS-independent signaling pathways.

Tue Sep 01 00:00:00 UTC 2015

3,3'-Diindolylmethane inhibits VEGF expression through the HIF-1α and NF-κB pathways in human retinal pigment epithelial cells under chemical hypoxic conditions.

International journal of molecular medicine

Park H,Lee DS,Yim MJ,Choi YH,Park S,Seo SK,Choi JS,Jang WH,Yea SS,Park WS,Lee CM,Jung WK,Choi IW

14-6731 was used in Electrophoretic mobility shift assay to demonstrate that DIM inhibits the CoCl2-induced production of VEGF by suppressing mitochondrial ROS production.

Wed Jul 01 00:00:00 UTC 2015

Down-regulation of IKKβ expression in glioma-infiltrating microglia/macrophages is associated with defective inflammatory/immune gene responses in glioblastoma.

Oncotarget

Mieczkowski J,Kocyk M,Nauman P,Gabrusiewicz K,Sielska M,Przanowski P,Maleszewska M,Rajan WD,Pszczolkowska D,Tykocki T,Grajkowska W,Kotulska K,Roszkowski M,Kostkiewicz B,Kaminska B

14-6731 was used in Immunocytochemistry to show that down-regulation of IKBKB and NFκB signaling in microglia/macrophages infiltrating glioblastoma correlates with defective expression of immune/inflammatory genes and M2 polarization.

Tue Oct 20 00:00:00 UTC 2015

Transmembrane peptides used to investigate the homo-oligomeric interface and binding hotspot of latent membrane protein 1.

Biopolymers

Sammond DW,Joce C,Takeshita R,McQuate SE,Ghosh N,Martin JM,Yin H

Tue Nov 01 00:00:00 UTC 2011

Knockout of the tumor suppressor gene Gprc5a in mice leads to NF-kappaB activation in airway epithelium and promotes lung inflammation and tumorigenesis.

Cancer prevention research (Philadelphia, Pa.)

Deng J,Fujimoto J,Ye XF,Men TY,Van Pelt CS,Chen YL,Lin XF,Kadara H,Tao Q,Lotan D,Lotan R

Thu Apr 01 00:00:00 UTC 2010

Knockout of the tumor suppressor gene Gprc5a in mice leads to NF-kappaB activation in airway epithelium and promotes lung inflammation and tumorigenesis.

Cancer prevention research (Philadelphia, Pa.)

Deng J,Fujimoto J,Ye XF,Men TY,Van Pelt CS,Chen YL,Lin XF,Kadara H,Tao Q,Lotan D,Lotan R

Thu Apr 01 00:00:00 UTC 2010

Knockout of the tumor suppressor gene Gprc5a in mice leads to NF-kappaB activation in airway epithelium and promotes lung inflammation and tumorigenesis.

Cancer prevention research (Philadelphia, Pa.)

Deng J,Fujimoto J,Ye XF,Men TY,Van Pelt CS,Chen YL,Lin XF,Kadara H,Tao Q,Lotan D,Lotan R

Thu Apr 01 00:00:00 UTC 2010

NF-kappaB activation through the alternative pathway correlates with chemoresistance and poor survival in extranodal NK/T-cell lymphoma, nasal type.

Japanese journal of clinical oncology

Liu X,Wang B,Ma X,Guo Y

14-6731 was used in Immunohistochemistry to investigate how NF-kappaB activation through the alternative pathway is frequently observed in NK/T-cell lymphoma and associated with chemoresistance and poor survival.

Wed Jul 01 00:00:00 UTC 2009

Alteration of NF-kappaB activity leads to mitochondrial apoptosis after infection with pathological prion protein.

Cellular microbiology

Bourteele S,Oesterle K,Weinzierl AO,Paxian S,Riemann M,Schmid RM,Planz O

Sat Sep 01 00:00:00 UTC 2007

Alteration of NF-kappaB activity leads to mitochondrial apoptosis after infection with pathological prion protein.

Cellular microbiology

Bourteele S,Oesterle K,Weinzierl AO,Paxian S,Riemann M,Schmid RM,Planz O

Sat Sep 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; EBP-1; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; nuclear factor kappa B subunit p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

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Gene Aliases: NFkB; NFKB3; p65; RELA

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UniProt ID: (Human) Q04206, (Mouse) Q04207

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Entrez Gene ID: (Human) 5970, (Mouse) 19697, (Rat) 309165

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding protein complex binding activating transcription factor binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding NF-kappaB binding repressing transcription factor binding ankyrin repeat binding enzyme binding sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFkB p65 Polyclonal Antibody, eBioscience™

NFkB p65 Antibody (14-6731-81) in ICC/IF

Product Details

14-6731-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Immunomicroscopy (IM)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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