Invitrogen

NFkB p65 Monoclonal Antibody (2A12A7)

NFkB p65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NFkB p65 Antibody (33-9900) in IHC (P)

Immunohistochemistry analysis of NF-kappa-B (p65) showing staining in the Cytoplasm and Nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More

NFkB p65 Antibody in Immunocytochemistry (ICC/IF)

NFkB p65 Antibody in Mass Spectrometry (MS)

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Product Details

33-9900

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 - 1 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

ChIP assay (ChIP)

3 µg/mL

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

Mass Spectrometry (MS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2A12A7

Immunogen

Recombinant protein (~175 amino acids) from the C-terminal portion of human NF-kappa-B (p65).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533153

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Efficacy of CU06-1004 via regulation of inflammation and endothelial permeability in LPS-induced acute lung injury.

Journal of inflammation (London, England)

Kim Y,Bae CR,Kim D,Kim H,Lee S,Zhang H,Noh M,Kim YM,Mochizuki N,Kwon YG

33-9900 was used in Immunohistochemistry to elucidate the effect of CU06-1004 in terms of prevention of inflammation and endothelial dysfunction in an ALI mouse model.

Thu Apr 06 00:00:00 UTC 2023

Efficacy of CU06-1004 via regulation of inflammation and endothelial permeability in LPS-induced acute lung injury.

Journal of inflammation (London, England)

Kim Y,Bae CR,Kim D,Kim H,Lee S,Zhang H,Noh M,Kim YM,Mochizuki N,Kwon YG

33-9900 was used in Immunohistochemistry to elucidate the effect of CU06-1004 in terms of prevention of inflammation and endothelial dysfunction in an ALI mouse model.

Thu Apr 06 00:00:00 UTC 2023

Impact of Roux-en-Y Gastric Bypass on Mitochondrial Biogenesis and Dynamics in Leukocytes of Obese Women.

Antioxidants (Basel, Switzerland)

Abad-Jiménez Z,Vezza T,López-Domènech S,Fernández-Reyes M,Canet F,Morillas C,Gómez-Abril SÁ,Bañuls C,Víctor VM,Rocha M

33-9900 was used in Western Blot to investigate whether RYGB modulates oxidative stress, inflammation and mitochondrial dynamics in the leukocytes of 47 obese women at one year follow-up.

Wed Jun 29 00:00:00 UTC 2022

Estrogen Receptor Beta Influences the Inflammatory p65 Cistrome in Colon Cancer Cells.

Frontiers in endocrinology

Indukuri R,Hases L,Archer A,Williams C

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in ChIP assay

Fri Jan 07 00:00:00 UTC 2022

NF-κB activation in retinal microglia is involved in the inflammatory and neovascularization signaling in laser-induced choroidal neovascularization in mice.

Experimental cell research

Hikage F,Lennikov A,Mukwaya A,Lachota M,Ida Y,Utheim TP,Chen DF,Huang H,Ohguro H

33-9900 was used in Immunohistochemistry to indicate that NF-κB activation in microglia and it's migration capacity is involved in the development of laser CNV in mice. Its suppression by NF-κB inhibition might be a promising therapeutic strategy for wet AMD.

Tue Jun 01 00:00:00 UTC 2021

Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression.

Molecular cell

Mejhert N,Kuruvilla L,Gabriel KR,Elliott SD,Guie MA,Wang H,Lai ZW,Lane EA,Christiano R,Danial NN,Farese RV,Walther TC

33-9900 was used in Immunocytochemistry-immunoflourescence to apply a genome-wide screening to identify genes that modulate lipid storage in macrophages, a cell type involved in metabolic diseases.

Thu Mar 19 00:00:00 UTC 2020

Epigenetic Suppression of HIV in Myeloid Cells by the BRD4-Selective Small Molecule Modulator ZL0580.

Journal of virology

Alamer E,Zhong C,Liu Z,Niu Q,Long F,Guo L,Gelman BB,Soong L,Zhou J,Hu H

33-9900 was used in Western Blotting to suggest a potential new approach that has utility in combination with ART to suppress residual HIV replication and/or HIV reactivation in CNS reservoirs, thereby reducing neuroinflammation and neurological disorders in HIV-infected individuals.

Mon May 18 00:00:00 UTC 2020

Cyclin F Downregulation Affects Epithelial-Mesenchymal Transition Increasing Proliferation and Migration of the A-375 Melanoma Cell Line.

Cancer management and research

Krajewski A,Gagat M,Mikołajczyk K,Izdebska M,Żuryń A,Grzanka A

33-9900 was used in Western Blotting to report that the downregulation of cyclin F in the A-375 melanoma cell line increases cell viability and colony formation in a cell cycle independent manner.

Tue Apr 19 00:00:00 UTC 2022

Structure-guided drug design identifies a BRD4-selective small molecule that suppresses HIV.

The Journal of clinical investigation

Niu Q,Liu Z,Alamer E,Fan X,Chen H,Endsley J,Gelman BB,Tian B,Kim JH,Michael NL,Robb ML,Ananworanich J,Zhou J,Hu H

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in Western Blot

Mon Jul 22 00:00:00 UTC 2019

Role of p62/SQSTM1 beyond autophagy: a lesson learned from drug-induced toxicity in vitro.

British journal of pharmacology

Alegre F,Moragrega ÁB,Polo M,Marti-Rodrigo A,Esplugues JV,Blas-Garcia A,Apostolova N

Published figure using NFkB p65 monoclonal antibody (Product # 33-9900) in Western Blot

Thu Feb 01 00:00:00 UTC 2018

A Single Nucleotide Polymorphism in the Vitamin D Receptor Gene Is Associated With Decreased Levels of the Protein and a Penetrating Pattern in Crohn's Disease.

Inflammatory bowel diseases

Gisbert-Ferrándiz L,Salvador P,Ortiz-Masiá D,Macías-Ceja DC,Orden S,Esplugues JV,Calatayud S,Hinojosa J,Barrachina MD,Hernández C

33-9900 was used in Western Blotting to analyze differences in vitamin D receptor expression levels among Crohn's Disease patients.

Fri Jun 08 00:00:00 UTC 2018

Glycocalyx Degradation Induces a Proinflammatory Phenotype and Increased Leukocyte Adhesion in Cultured Endothelial Cells under Flow.

PloS one

McDonald KK,Cooper S,Danielzak L,Leask RL

33-9900 was used in Immunocytochemistry-immunoflourescence to show that destabilisation of endothelial glycocalyx may be an early and defining feature in the initiation of atherosclerosis.

Fri Jun 30 00:00:00 UTC 2017

Antipsychotic drugs suppress the AKT/NF-κB pathway and regulate the differentiation of T-cell subsets.

Immunology letters

Chen ML,Tsai TC,Lin YY,Tsai YM,Wang LK,Lee MC,Tsai FM

Sun Oct 30 00:00:00 UTC 2011

Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies.

Journal of neuroinflammation

Herkenham M,Rathore P,Brown P,Listwak SJ

33-9900 was used in immunocytochemistry, immunohistochemistry (free floating), and western blot to examine specificity of the anti-NF-κB antibodies.

Fri Oct 14 00:00:00 UTC 2011

Sequence characteristics of functional siRNAs.

RNA (New York, N.Y.)

Jagla B,Aulner N,Kelly PD,Song D,Volchuk A,Zatorski A,Shum D,Mayer T,De Angelis DA,Ouerfelli O,Rutishauser U,Rothman JE

33-9900 was used in immunocytochemistry to develop an algorithm to generate effective siRNAs

Wed Jun 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; EBP-1; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

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Gene Aliases: NFKB3; p65; RELA

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UniProt ID: (Human) Q04206, (Mouse) Q04207

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Entrez Gene ID: (Human) 5970, (Mouse) 19697

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding protein complex binding activating transcription factor binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding NF-kappaB binding repressing transcription factor binding ankyrin repeat binding enzyme binding sequence-specific DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFkB p65 Monoclonal Antibody (2A12A7)

NFkB p65 Antibody (33-9900) in IHC (P)

Product Details

33-9900

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Gel Shift (GS)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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