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Invitrogen
NG2 Polyclonal Antibody
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FIGURE: 1 / 5
NG2 Antibody (PA5-100235) in ICC/IF
Immunofluorescent analysis of NG2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and NG2 polyclonal antibody (Product # PA5-100235) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100235
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CSPG4(Accession Q6UVK1), corresponding to amino acid residues P1840-P1890.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815765
Product Specific Information
Antibody detects endogenous levels of total NG2.
Target Information
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AN2 proteoglycan; AN2/NG2 proteoglycan; Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; HMW MAA; HSN tumor-specific antigen; MEL CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; membrane-spanning proteoglycan NG2; nerve/glial antigen 2; Proteoglycan AN2
Gene Aliases: 4732461B14Rik; AN2; CSPG4; HMW-MAA; Kiaa4232; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
UniProt ID: (Human) Q6UVK1, (Rat) Q00657, (Mouse) Q8VHY0
Entrez Gene ID: (Human) 1464, (Rat) 81651, (Mouse) 121021
activation of MAPK activity
angiogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
glial cell migration
cell migration
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
intracellular signal transduction
tissue remodeling
positive regulation of peptidyl-tyrosine phosphorylation
inflammatory response
signal transduction
response to lithium ion
negative regulation of neuron projection development
neuron remodeling
multicellular organism development
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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