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Invitrogen
NGAL Polyclonal Antibody
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NGAL Antibody (PA5-79591) in IHC (P)
Immunohistochemistry analysis of NGAL on paraffin-embedded mouse spleen tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with NGAL polyclonal antibody (Product# PA5-79591) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Developme... View More
Product Details
PA5-79591
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746706
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Mouse Lung Tissue, Mouse Intestine Tissue. IHC: Mouse Spleen tissue, Rat Spleen tissue IHC-F: mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue.
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; HGNC:6526; lipocalin 2 (oncogene 24p3); Lipocalin 24p3; Lipocalin-2; Lipocalin2; migration-stimulating factor inhibitor; Neutrophil gelatinase-associated lipocalin; NGAL; Oncogene 24p3; OTTHUMP00000022240; p25; RP23-161B9.11; secreted inducible protein 24; Siderocalin; Siderocalin LCN2; SV-40-induced 24P3 protein; uterocalin
Gene Aliases: 24p3; AW212229; HNL; LCN2; MSFI; NGAL; p25; Sip24
UniProt ID: (Human) P80188, (Mouse) P11672, (Rat) P30152
Entrez Gene ID: (Human) 3934, (Mouse) 16819, (Rat) 170496
immune system process
transport
ion transport
apoptotic process
response to virus
response to herbicide
positive regulation of gene expression
siderophore transport
positive regulation of cell projection organization
response to drug
innate immune response
iron ion homeostasis
protein homotrimerization
cellular response to hydrogen peroxide
extrinsic apoptotic signaling pathway in absence of ligand
response to oxidative stress
response to bacterium
response to mycotoxin
response to nutrient levels
cellular response to nutrient levels
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular iron ion homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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