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Invitrogen
NGEF Polyclonal Antibody
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FIGURE: 1 / 1
NGEF Antibody (PA5-110028) in WB
Western blot analysis of NGEF in extracts of various cell lines. Samples were incubated with NGEF Polyclonal antibody (Product # PA5-110028) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
PA5-110028
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 429-710 of human NGEF (NP_062824.2).
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855439
Product Specific Information
Immunogen sequence: RVEERSEREC TALDAHKELE MVVKACNEGV RKMSRTEQMI SIQKKMEFKI KSVPIISHSR WLLKQGELQQ MSGPKTSRTL RTKKLFHEIY LFLFNDLLVI CRQIPGDKYQ VFDSAPRGLL RVEELEDQGQ TLANVFILRL LENADDREAT YMLKASSQSE MKRWMTSLAP NRRTKFVSFT SRLLDCPQVQ CVHPYVAQQP DELTLELADI LNILDKTDDG WIFGERLHDQ ERGWFPSSMT EEILNPKIRS QNLKECFRVH KMDDPQRSQN KDRRKLGSRN RQ
Target Information
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain-enriched SH3-domain protein Besh3; Eph-interacting exchange protein; Ephexin-1; Neuronal guanine nucleotide exchange factor; transforming immortalised mammary epithelial sequence 2
Gene Aliases: ARHGEF27; Besh3; EPHEXIN; NGEF; Tims2
UniProt ID: (Human) Q8N5V2, (Mouse) Q8CHT1
Entrez Gene ID: (Human) 25791, (Mouse) 53972, (Rat) 246217
multicellular organism development
nervous system development
cellular process
cell differentiation
regulation of Rho protein signal transduction
regulation of GTPase activity
positive regulation of GTPase activity
ephrin receptor signaling pathway
negative regulation of dendritic spine morphogenesis
positive regulation of apoptotic process
regulation of small GTPase mediated signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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