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Invitrogen
NGF Recombinant Rabbit Monoclonal Antibody (R711)
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NGF Antibody (MA5-29443) in Neu
Neutralization of Cell Proliferation Induced by beta-NGF. Recombinant Human beta-NGF stimulates proliferation in the TF1 human erythroleukemic cell line. Proliferation elicited by Recombinant Human beta-NGF (10 ng/mL) is neutralized by increasing concentrations of NGF Recombinant Rabbit Monoclonal Antibody (R711) (Product # MA5-29443). The IC50 is typically 19 µg/mL.
Product Details
MA5-29443
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
R711
Immunogen
Recombinant Human NGF/NGFB Protein (Ser122-Arg239)
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
Histidine/arginine, pH 6, with 120mM NaCl, 0.02% Tween 80
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785305
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human NGF/NGFB.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B nerve growth factor; b-NGF; beta ngf; Beta-nerve growth factor; Beta-NGF; BetaNGF; bNGF; MGC161426; MGC161428; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGF b; NGF β; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
Gene Aliases: Beta-NGF; HSAN5; NGF; NGFB
UniProt ID: (Human) P01138
Entrez Gene ID: (Human) 4803
activation of MAPKK activity
activation of cysteine-type endopeptidase activity involved in apoptotic process
microtubule-based movement
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of gene expression
nerve growth factor processing
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of neuron apoptotic process
regulation of neuron differentiation
positive regulation of Ras protein signal transduction
neurotrophin TRK receptor signaling pathway
phosphatidylinositol-mediated signaling
neuron projection morphogenesis
positive regulation of axonogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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