Invitrogen

NGFR Monoclonal Antibody (NGFR5)

NGFR Antibody in Immunocytochemistry (ICC/IF)

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NGFR Antibody (MA5-13314) in ICC/IF

Immunofluorescence analysis of NGFR was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NGFR Monoclonal Antibody (NGFR5) (Product # MA5-13314) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766)... View More

NGFR Antibody in Immunohistochemistry (IHC)

Product Image Gallery

NGFR Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-NGFR Monoclonal Antibody (NGFR5) (Product # MA5-13314), shows upregulation of NGFR expression upon neuronal differentiation of SH-SY5Y cell line using retinoic acid. {RE}

Product Details

MA5-13314

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10-1:200

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

1:10-1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

2 µg/test

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Cat, Ferret, Human, Non-human primate, Rabbit, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

NGFR5

Immunogen

NGFR from A875 melanoma cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10982037

Product Specific Information

MA5-13314 targets Neurotrophin Receptor /NGF-Receptor (p75NGFR) in FACS, ICC/IF and WB applications and shows reactivity with Feline, Ferret, Human, Rat, Non-human primate, and Rabbit samples. This antibody does not react with rat tissue in Western blot applications.

The MA5-13314 immunogen is nGFR from A875 melanoma cells.

Target Information

Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A genetic compensatory mechanism regulated by Jun and Mef2d modulates the expression of distinct class IIa Hdacs to ensure peripheral nerve myelination and repair.

eLife

Velasco-Aviles S,Patel N,Casillas-Bajo A,Frutos-Rincón L,Velasco E,Gallar J,Arthur-Farraj P,Gomez-Sanchez JA,Cabedo H

MA5-13314 was used in Immunohistochemistry to show that a transcription factor regulated genetic compensatory mechanism within this family of proteins, blocks negative regulators of myelination ensuring peripheral nerve developmental myelination and remyelination after injury.

Tue Jan 25 00:00:00 UTC 2022

The Effect of Interaction NGF/p75^NTR in Sperm Cells: A Rabbit Model.

Cells

Castellini C,Mattioli S,Cotozzolo E,Pistilli A,Rende M,Bartolini D,Di Sante G,Menchetti L,Dal Bosco A,Stabile AM

MA5-13314 was used in Flow cytometry/Cell sorting to analyze the impact of NGF p75NTR in epididymal and ejaculated rabbit semen during in vitro sperm storage.

Fri Mar 18 00:00:00 UTC 2022

Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells.

Cell stem cell

Hawkins FJ,Suzuki S,Beermann ML,Barillà C,Wang R,Villacorta-Martin C,Berical A,Jean JC,Le Suer J,Matte T,Simone-Roach C,Tang Y,Schlaeger TM,Crane AM,Matthias N,Huang SXL,Randell SH,Wu J,Spence JR,Carraro G,Stripp BR,Rab A,Sorsher EJ,Horani A,Brody SL,Davis BR,Kotton DN

MA5-13314 was used in Immunohistochemistry to report the directed differentiation of human iPSCs into airway basal cells ("iBCs"), a population resembling the stem cell of the airway epithelium.

Thu Jan 07 00:00:00 UTC 2021

Exosomes Produced by Mesenchymal Stem Cells Drive Differentiation of Myeloid Cells into Immunosuppressive M2-Polarized Macrophages in Breast Cancer.

Journal of immunology (Baltimore, Md. : 1950)

Biswas S,Mandal G,Roy Chowdhury S,Purohit S,Payne KK,Anadon C,Gupta A,Swanson P,Yu X,Conejo-Garcia JR,Bhattacharyya A

Published figure using NGFR monoclonal antibody (Product # MA5-13314) in Immunocytochemistry

Sun Dec 15 00:00:00 UTC 2019

Reduced variability of neural progenitor cells and improved purity of neuronal cultures using magnetic activated cell sorting.

PloS one

Bowles KR,Tcw J,Qian L,Jadow BM,Goate AM

MA5-13314 was used in Immunocytochemistry-immunoflourescence to develop a technique for incorporation into standard neural progenitor cell differentiation and maintenance protocols in order to improve culture homogeneity and consistency.

Fri Dec 06 00:00:00 UTC 2019

In vitro effect of nerve growth factor on the main traits of rabbit sperm.

Reproductive biology and endocrinology : RB&E

Castellini C,Mattioli S,Dal Bosco A,Collodel G,Pistilli A,Stabile AM,Macchioni L,Mancuso F,Luca G,Rende M

MA5-13314 was used in Immunocytochemistry-immunoflourescence to provide new insights on human fertility through the exploration of nerve growth factor effects on rabbit sperm functions in vitro.

Tue Nov 12 00:00:00 UTC 2019

A phase1 study of stereotactic gene delivery of AAV2-NGF for Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Rafii MS,Baumann TL,Bakay RA,Ostrove JM,Siffert J,Fleisher AS,Herzog CD,Barba D,Pay M,Salmon DP,Chu Y,Kordower JH,Bishop K,Keator D,Potkin S,Bartus RT

MA5-13314 was used in immunohistochemistry to perform a phase I clinical trial to determine the safety and tolerability of an AAV2-NGF vector intended for therapy of Alzheimer's disease

Mon Sep 01 00:00:00 UTC 2014

Rac1b increases with progressive tau pathology within cholinergic nucleus basalis neurons in Alzheimer's disease.

The American journal of pathology

Perez SE,Getova DP,He B,Counts SE,Geula C,Desire L,Coutadeur S,Peillon H,Ginsberg SD,Mufson EJ

MA5-13314 was used in immunohistochemistry to study the expression of Raclb in cholinergic nucleus basalis neurones and the correlation with tau pathology and Alzheimer's disease progression

Wed Feb 01 00:00:00 UTC 2012

Human neurotrophin receptor p75NTR defines differentiation-oriented skeletal muscle precursor cells: implications for muscle regeneration.

Journal of neuropathology and experimental neurology

Colombo E,Romaggi S,Medico E,Menon R,Mora M,Falcone C,Lochmüller H,Confalonieri P,Mantegazza R,Morandi L,Farina C

MA5-13314 was used in blocking assay to study the expression and function of p75NTR in human skeletal muscle precursor cells.

Tue Feb 01 00:00:00 UTC 2011

Low-affinity nerve growth factor receptor (P75 NGFR) as a marker of perineural invasion in malignant melanomas.

Journal of cutaneous pathology

Chan MM,Tahan SR

MA5-13314 was used in immunohistochemistry to evaluate low-affinity nerve growth factor receptor as a biomarker of perineural invasion in cutaneous neoplasms

Mon Mar 01 00:00:00 UTC 2010

In situ evidence of involvement of Schwann cells in ulcerative colitis: autocrine and paracrine signaling by A disintegrin and metalloprotease-17-mediated tumor necrosis factor alpha production.

Human pathology

Mosnier JF,Jarry A,Camdessanché JP,Antoine JC,Laboisse CL

MA5-13314 was used in immunohistochemistry to investigate the involvement of S100 protein-positive cells in ulcerative colitis

Sat Aug 01 00:00:00 UTC 2009

Immunohistochemical expression patterns of neural and neuroendocrine markers, the neural growth factor receptors and the beta-tubulin II and IV isotypes in human thymus.

Anticancer research

Bai M,Papoudou-Bai A,Karatzias G,Doukas M,Goussia A,Stefanaki K,Rontogianni D,Dalavanga Y,Agnantis NJ,Kanavaros P

MA5-13314 was used in immunohistochemistry to study the expression of the neural growth factor receptors and beta-tubulin II and IV isotypes in human thymus

Thu Apr 10 00:00:00 UTC 2008

Immunophenotypical pleomorphism expression in sudden cardiac death.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Ceauşu M,Curcă C,Ardeleanu C,Dermengiu D

MA5-13314 was used in immunohistochemistry to investigate the expression of some histopathological and immunohistochemical markers in sudden cardiac death

Thu Dec 11 00:00:00 UTC 2008

The activated nerve growth factor receptor p-TrkA is selectively expressed in advanced-stage ovarian carcinoma.

Human pathology

Ødegaard E,Staff AC,Abeler VM,Kopolovic J,Onsrud M,Lazarovici P,Davidson B

MA5-13314 was used in immunohistochemistry to study the expression of the activated nerve growth factor receptor p-TrkA in advanced-stage ovarian carcinoma

Mon Jan 01 00:00:00 UTC 2007

Immunohistochemical localization of epithelial rests of Malassez in human periodontal membrane.

European journal of orthodontics

Becktor KB,Nolting D,Becktor JP,Kjaer I

MA5-13314 was used in immunohistochemistry to identify epithelial rests of Malassez in human periodontal membrane

Wed Aug 01 00:00:00 UTC 2007

Origin and ontogeny of mammalian ovarian neurons.

Endocrinology

Dees WL,Hiney JK,McArthur NH,Johnson GA,Dissen GA,Ojeda SR

MA5-13314 was used in immunohistochemistry to examine the neurogenesis in mammalian ovaries

Tue Aug 01 00:00:00 UTC 2006

The p75 neurotrophin receptor is widely expressed in conventional papillary thyroid carcinoma.

Human pathology

Rocha AS,Risberg B,Magalhães J,Trovisco V,de Castro IV,Lazarovici P,Soares P,Davidson B,Sobrinho-Simões M

MA5-13314 was used in immunoprecipitation to study the expression of the p75 neurotrophin receptor in conventional papillary thyroid carcinoma

Mon May 01 00:00:00 UTC 2006

Differential expression of the neurotrophin receptors p75NTR, TrkA, TrkB and TrkC in human peripheral blood mononuclear cells.

Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie

Nassenstein C,Möhring UH,Luttmann W,Virchow JC,Braun A

MA5-13314 was used in flow cytometry to study the expression of neurotrophin receptors in human peripheral blood mononuclear cells

Thu Jun 01 00:00:00 UTC 2006

A histological and anatomical profile of pacinian corpuscles from Dupuytren's contracture and the expression of nerve growth factor receptor.

Plastic and reconstructive surgery

Ehrmantant WR,Graham WP,Towfighi J,Mackay DR,Ehrlich HP

MA5-13314 was used in immunohistochemistry to study the role of nerve growth factor in the growth of pacinian corpuscles in Dupuytren's disease

Wed Sep 01 00:00:00 UTC 2004

The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.

The Journal of experimental medicine

Nassenstein C,Braun A,Erpenbeck VJ,Lommatzsch M,Schmidt S,Krug N,Luttmann W,Renz H,Virchow JC

MA5-13314 was used in immunocytochemistry to study the role of neurotrophins as survival and activation factors for eosinophils in allergic bronchial asthma

Mon Aug 04 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: CD271; CD271 antigen; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Low-affinity nerve growth factor receptor p75NGFR; Low-affinity nerve growth factor receptor p75NGR; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor, fast; NGF receptor; p-75; p75 ICD; p75 ntr; TNFR superfamily, member 16; TNR16; Tumor necrosis factor receptor superfamily member 16

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Gene Aliases: CD271; Gp80-LNGFR; LNGFR; NGFR; p75; p75(NTR); p75NTR; RNNGFRR; TNFRSF16

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UniProt ID: (Human) P08138, (Rat) P07174

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Entrez Gene ID: (Human) 4804, (Rat) 24596

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Function(s)

beta-amyloid binding signal transducer activity receptor activity transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity neurotrophin TRKA receptor binding protein binding Rab GTPase binding ubiquitin protein ligase binding nerve growth factor binding preprotein binding death receptor activity neurotrophin binding

Process(es)

Performance Guarantee

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NGFR Monoclonal Antibody (NGFR5)

NGFR Antibody (MA5-13314) in ICC/IF

Product Details

MA5-13314

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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