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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • NGFR (CD271) Antibodies

          Invitrogen

          NGFR Monoclonal Antibody (NGFR5)

          Advanced Verification
          4 Published Figures
          20 References
          View all (100) NGFR (CD271) antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite NGFR Monoclonal Antibody (NGFR5)

          • Antibody Testing Data (7)
          • Published Figures (4)
          • Advanced Verification (1)
          NGFR Antibody in Immunocytochemistry (ICC/IF)
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          NGFR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          NGFR Antibody (MA5-13314) in ICC/IF

          Immunofluorescence analysis of NGFR was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NGFR Monoclonal Antibody (NGFR5) (Product # MA5-13314) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766)... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          NGFR Antibody in Immunocytochemistry (ICC/IF)
          NGFR Antibody in Immunocytochemistry (ICC/IF)
          NGFR Antibody in Western Blot (WB)
          NGFR Antibody in Western Blot (WB)
          NGFR Antibody in Flow Cytometry (Flow)
          NGFR Antibody in Flow Cytometry (Flow)
          NGFR Antibody in Flow Cytometry (Flow)
          NGFR Antibody in Immunocytochemistry (ICC/IF)
          NGFR Antibody in Immunocytochemistry (ICC/IF)
          NGFR Antibody in Immunocytochemistry (ICC/IF)
          NGFR Antibody in Immunohistochemistry (IHC)
          NGFR Antibody

          Product Details

          MA5-13314

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:10-1:200
          -

          Immunohistochemistry (IHC)

          -
          View 12 publications 12 publications

          Immunocytochemistry (ICC/IF)

          1:10-1:200
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          2 µg/test
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Cat, Ferret, Human, Non-human primate, Rabbit, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          NGFR5

          Immunogen

          NGFR from A875 melanoma cells
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4, with 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10982037

          Product Specific Information

          MA5-13314 targets Neurotrophin Receptor /NGF-Receptor (p75NGFR) in FACS, ICC/IF and WB applications and shows reactivity with Feline, Ferret, Human, Rat, Non-human primate, and Rabbit samples. This antibody does not react with rat tissue in Western blot applications.

          The MA5-13314 immunogen is nGFR from A875 melanoma cells.

          Target Information

          Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD271; CD271 antigen; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Low-affinity nerve growth factor receptor p75NGFR; Low-affinity nerve growth factor receptor p75NGR; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor, fast; NGF receptor; p-75; p75 ICD; p75 ntr; TNFR superfamily, member 16; TNR16; Tumor necrosis factor receptor superfamily member 16

          View more View less

          Gene Aliases: CD271; Gp80-LNGFR; LNGFR; NGFR; p75; p75(NTR); p75NTR; RNNGFRR; TNFRSF16

          View more View less

          UniProt ID: (Human) P08138, (Rat) P07174

          View more View less

          Entrez Gene ID: (Human) 4804, (Rat) 24596

          View more View less

          Function(s)
          beta-amyloid binding signal transducer activity receptor activity transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity neurotrophin TRKA receptor binding protein binding Rab GTPase binding ubiquitin protein ligase binding nerve growth factor binding preprotein binding death receptor activity neurotrophin binding
          Process(es)
          intracellular protein transport apoptotic process signal transduction axon guidance central nervous system development response to wounding regulation of gene expression positive regulation of cell death negative regulation of neuron projection development programmed cell death detection of temperature stimulus sensory perception of pain nerve development hair follicle morphogenesis positive regulation of myelination positive regulation of synaptic transmission, cholinergic response to lipopolysaccharide circadian regulation of gene expression cellular response to oxidative stress positive regulation of Rho protein signal transduction skeletal muscle cell differentiation negative regulation of fibroblast growth factor receptor signaling pathway positive regulation of odontogenesis of dentin-containing tooth glucose homeostasis positive regulation of apoptotic process positive regulation of MAPK cascade negative regulation of neuron apoptotic process skin development positive regulation of fibroblast proliferation negative regulation of hair follicle development positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization positive regulation of synaptic transmission, glutamatergic negative regulation of cell death positive regulation of neuron death regulation of reactive oxygen species metabolic process positive regulation of apoptotic signaling pathway regulation of glucose import in response to insulin stimulus activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response immune response membrane protein intracellular domain proteolysis tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process neurotrophin TRK receptor signaling pathway negative regulation of axonogenesis positive regulation of axonogenesis apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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