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Alomone Labs, LTD
NHERF-1/EBP50 Polyclonal Antibody
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Product Details
APZ-006-200UL
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1, Between the PDZ2 and the ERM binding domains.
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 5% sucrose, 1% BSA
Contains
0.025% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EBP50; ERM-binding phosphoprotein; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1
Gene Aliases: Nherf; Nherf1; Slc9a3r1
UniProt ID: (Rat) Q9JJ19
Entrez Gene ID: (Rat) 59114
receptor binding
protein binding
beta-catenin binding
chloride channel regulator activity
phosphatase binding
protein domain specific binding
PDZ domain binding
beta-2 adrenergic receptor binding
protein complex binding
protein complex scaffold
protein self-association
dopamine receptor binding
growth factor receptor binding
renal sodium ion transport
adenylate cyclase-activating dopamine receptor signaling pathway
sensory perception of sound
negative regulation of cell proliferation
negative regulation of platelet-derived growth factor receptor signaling pathway
negative regulation of sodium ion transport
negative regulation of phosphatidylinositol 3-kinase signaling
Wnt signaling pathway
cAMP-mediated signaling
microvillus assembly
actin cytoskeleton organization
negative regulation of cell migration
cellular phosphate ion homeostasis
negative regulation of sodium:proton antiporter activity
bile acid secretion
cellular protein localization
glutathione transport
regulation of excretion
regulation of protein kinase activity
negative regulation of protein kinase B signaling
auditory receptor cell stereocilium organization
phospholipase C-activating dopamine receptor signaling pathway
renal absorption
negative regulation of ERK1 and ERK2 cascade
renal phosphate ion absorption
negative regulation of cell motility
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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