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NHERF2 Antibody (PA5-110395) in WB
Western blot analysis of NHERF2 in extracts of HeLa cells. Samples were incubated with NHERF2 Polyclonal antibody (Product # PA5-110395) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-110395
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 180-300 of human SLC9A3R2 (NP_001123484).
Conjugate
Form
Liquid
Concentration
0.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855806
Product Specific Information
Immunogen sequence: DPGSPAARSG LRAQDRLIEV NGQNVEGLRH AEVVASIKAR EDEARLLVVD PETDEHFKRL RVTPTEEHVE GPLPSPVTNG TSPAQLNGGS ACSSRSDLPG SDKDTEDGSA WKQDPFQESG L
Target Information
NHE3 is a sodium/hydrogen exchanger in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 and protein kinase A phosphorylation, may play a role in NHE3 regulation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:11076; MGC104639; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 kinase A regulatory protein E3KARP; NHE3 regulatory factor 2; NHERF-2; SIP-1; Sodium-hydrogen exchanger regulatory factor 2; sodium/hydrogen exchanger 3 kinase A regulatory protein; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2; Solute carrier family 9 isoform A3 regulatory factor 2; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2; SRY-interacting protein 1; TKA-1; Tyrosine kinase activator protein 1
Gene Aliases: E3KARP; NHE3RF2; NHERF-2; NHERF2; OCTS2; SIP-1; SIP1; SLC9A3R2; TKA-1
UniProt ID: (Human) Q15599
Entrez Gene ID: (Human) 9351
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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