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          • Primary Antibodies ›
          • NIT2 Antibodies

          Proteintech

          NIT2 Monoclonal Antibody (1E5B1)

          View all (26) NIT2 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite NIT2 Monoclonal Antibody (1E5B1)

          • Antibody Testing Data (8)
          NIT2 Antibody in Immunocytochemistry (ICC/IF)
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          NIT2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          NIT2 Antibody (68169-1-IG) in ICC/IF

          Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          NIT2 Antibody in Immunocytochemistry (ICC/IF)
          NIT2 Antibody in Immunocytochemistry (ICC/IF)
          NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NIT2 Antibody in Western Blot (WB)
          NIT2 Antibody in Western Blot (WB)
          NIT2 Antibody in Western Blot (WB)

          Product Details

          68169-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000-1:50,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:800
          -

          Immunocytochemistry (ICC/IF)

          1:200-1:800
          -

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rat

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1E5B1

          Immunogen

          Recombinant protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite Plus 488
          • Request custom conjugation

          Form

          Liquid

          Purification

          Protein A

          Storage buffer

          PBS with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice

          Product Specific Information

          Immunogen sequence: MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG AKYFPEYAEK IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF GPDGTLLAKY RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL GICYDMRFAE LAQIYAQRGC QLLVYPGAFN LTTGPAHWEL LQRSRAVDNQ VYVATASPAR DDKASYVAWG HSTVVNPWGE ALAKAGTEEA IVYSDIDLKK LAEIRQQIPV FRQKRSDLYA VEMKKP

          Target Information

          Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: epididymis secretory sperm binding protein Li 8a; Nit protein 2; nitrilase family, member 2; Nitrilase homolog 2; Omega-amidase NIT2

          View more View less

          Gene Aliases: 1190017B19Rik; CUA002; D16Ertd502e; HEL-S-8a; NIT2; RGD1310494

          View more View less

          UniProt ID: (Human) Q9NQR4, (Rat) Q497B0, (Mouse) Q9JHW2

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          Entrez Gene ID: (Human) 56954, (Pig) 100625406, (Rat) 288174, (Mouse) 52633

          View more View less

          Function(s)
          omega-amidase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
          Process(es)
          oxaloacetate metabolic process asparagine metabolic process glutamine metabolic process nitrogen compound metabolic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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