Invitrogen

NK1.1 Monoclonal Antibody (PK136)

Product Details

MA5-16916

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5-10 µg/mL

View 19 publications 19 publications

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PK136

Immunogen

Spleen and bone marrow cells from CE mice

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538393

Product Specific Information

This antibody does not react with human or rat.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Mouse CD161 / NK1.1 antibody, clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family.

Target Information

NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Combined blockade of the IL-13 and IL-33 pathways leads to a greater inhibition of type 2 inflammation over inhibition of either pathway alone.

The Journal of allergy and clinical immunology

Ramirez-Carrozzi V,Sambandam A,Zhou M,Yan D,Kang J,Wu X,Suto E,Baca M,Austin C,Xu M,Lee W,Pappu R

MA516916 was used in flow cytometry to study the effect of IL-13 and IL-33 pathways in dysregulated type 2 inflammation.

Wed Feb 01 00:00:00 UTC 2017

Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity.

Proceedings of the National Academy of Sciences of the United States of America

Li M,Li Z,Yao Y,Jin WN,Wood K,Liu Q,Shi FD,Hao J

MA516916 was used in flow cytometry to discuss the contribution of astrocytic IL-15 to postischemic brain damage

Tue Jan 17 00:00:00 UTC 2017

Glucocorticoid receptor in T cells mediates protection from autoimmunity in pregnancy.

Proceedings of the National Academy of Sciences of the United States of America

Engler JB,Kursawe N,Solano ME,Patas K,Wehrmann S,Heckmann N,Lühder F,Reichardt HM,Arck PC,Gold SM,Friese MA

MA516916 was used in flow cytometry to characterize conventional and regulatory T cells during pregnancy

Tue Jan 10 00:00:00 UTC 2017

NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury.

Journal of leukocyte biology

Hoegl S,Ehrentraut H,Brodsky KS,Victorino F,Golden-Mason L,Eltzschig HK,McNamee EN

MA516916 was used in flow cytometry to elucidate the role of Tbet in acute lung injury

Wed Feb 01 00:00:00 UTC 2017

Smad3 promotes cancer progression by inhibiting E4BP4-mediated NK cell development.

Nature communications

Tang PM,Zhou S,Meng XM,Wang QM,Li CJ,Lian GY,Huang XR,Tang YJ,Guan XY,Yan BP,To KF,Lan HY

MA516916 was used in flow cytometry to demonstrate that Smad3 in the tumor microenvironment regulates cancer progression

Mon Mar 06 00:00:00 UTC 2017

ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content.

Scientific reports

Nowyhed HN,Chandra S,Kiosses W,Marcovecchio P,Andary F,Zhao M,Fitzgerald ML,Kronenberg M,Hedrick CC

MA516916 was used in flow cytometry to show that ABCA7 regulates natural killer T cell development in a cell-extrinsic manner

Mon Jan 16 00:00:00 UTC 2017

Targeted antibody-mediated depletion of murine CD19 CAR T cells permanently reverses B cell aplasia.

The Journal of clinical investigation

Paszkiewicz PJ,Fräßle SP,Srivastava S,Sommermeyer D,Hudecek M,Drexler I,Sadelain M,Liu L,Jensen MC,Riddell SR,Busch DH

MA516916 was used in flow cytometry to demonstrate that targeting a truncated version of the epidermal growth factor receptor with cetuximab eliminates CD19 chimeric antigen receptor T cells

Tue Nov 01 00:00:00 UTC 2016

Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.

The Journal of experimental medicine

Henry EK,Sy CB,Inclan-Rico JM,Espinosa V,Ghanny SS,Dwyer DF,Soteropoulos P,Rivera A,Siracusa MC

MA516916 was used in flow cytometry to find a role for Car enzymes in regulating mast cell lineage commitment

Mon Aug 22 00:00:00 UTC 2016

Differential Role of Hematopoietic and Nonhematopoietic Cell Types in the Regulation of NK Cell Tolerance and Responsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Shifrin NT,Kissiov DU,Ardolino M,Joncker NT,Raulet DH

MA516916 was used in flow cytometry to study the role of MCH I in natural killer cell tolerance and hyporesponsiveness

Tue Nov 15 00:00:00 UTC 2016

CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control.

Oncoimmunology

Veinotte L,Gebremeskel S,Johnston B

MA516916 was used in flow cytometry to study the contribution of CXCR6/CXCL16 interactions in glycolipid-dependent invariant natural killer cell activation and tumor control

Wed Jun 01 00:00:00 UTC 2016

DNAM-1 controls NK cell activation via an ITT-like motif.

The Journal of experimental medicine

Zhang Z,Wu N,Lu Y,Davidson D,Colonna M,Veillette A

MA5-16916 was used in flow cytometry to study how DNAM-1 controls NK cell-mediated cytotoxicity and cytokine production

Mon Nov 16 00:00:00 UTC 2015

A novel Cd8-cis-regulatory element preferentially directs expression in CD44hiCD62L+ CD8+ T cells and in CD8αα+ dendritic cells.

Journal of leukocyte biology

Sakaguchi S,Hombauer M,Hassan H,Tanaka H,Yasmin N,Naoe Y,Bilic I,Moser MA,Hainberger D,Mayer H,Seiser C,Bergthaler A,Taniuchi I,Ellmeier W

MA5-16916 was used in ChIP assay to identify Cd8 cis-regulatory elements

Wed Apr 01 00:00:00 UTC 2015

Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis.

Development (Cambridge, England)

Reeh KA,Cardenas KT,Bain VE,Liu Z,Laurent M,Manley NR,Richie ER

MA5-16916 was used in flow cytometry to assess the contribution of Tbx1 to thymus and parathyroid development

Fri Aug 01 00:00:00 UTC 2014

Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin.

Blood

Jeannet G,Scheller M,Scarpellino L,Duboux S,Gardiol N,Back J,Kuttler F,Malanchi I,Birchmeier W,Leutz A,Huelsken J,Held W

MA5-16916 was used in flow cytometry to investigate whether hematopoietic cells transduce canonical Wnt signals in the absence of beta- and gamma-catenin

Tue Jan 01 00:00:00 UTC 2008

Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.

Cell and tissue research

Kanwar N,Fayyazi A,Backofen B,Nitsche M,Dressel R,von Mollard GF

MA5-16916 was used in flow cytometry to elucidate the role of VAMP8 in the thymus

Sat Nov 01 00:00:00 UTC 2008

T-bet concomitantly controls migration, survival, and effector functions during the development of Valpha14i NKT cells.

Blood

Matsuda JL,Zhang Q,Ndonye R,Richardson SK,Howell AR,Gapin L

MA5-16916 was used in flow cytometry to examine the role of T-bet in Valpha14i natural killer T cell function

Sat Apr 01 00:00:00 UTC 2006

A comparison of murine T-cell-depleted adult bone marrow and full-term fetal blood cells in hematopoietic engraftment and immune reconstitution.

Blood

Chen BJ,Cui X,Sempowski GD,Gooding ME,Liu C,Haynes BF,Chao NJ

MA5-16916 was used in flow cytometry to compare myeloid and lymphoid recovery using bone marrow and cord blood as sources of stem cells.

Tue Jan 01 00:00:00 UTC 2002

MHC class I molecules on adenovirus E1A-expressing tumor cells inhibit NK cell killing but not NK cell-mediated tumor rejection.

International immunology

Routes JM,Ryan JC,Ryan S,Nakamura M

MA5-16916 was used in flow cytometry to investigate the capacity of E1A to sensitize cells to multiple NK cell killing mechanisms

Mon Oct 01 00:00:00 UTC 2001

Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function.

Journal of immunology (Baltimore, Md. : 1950)

Carlyle JR,Martin A,Mehra A,Attisano L,Tsui FW,Zúñiga-Pflücker JC

MA5-16916 was used in flow cytometry and immunoprecipitation to identify NKR-P1B as a NK1.1 antigen

Sat May 15 00:00:00 UTC 1999

Natural killer cell proliferation induced by anti-NK1.1 and IL-2.

Immunology and cell biology

Reichlin A,Yokoyama WM

MA5-16916 was used in flow cytometry to report that immobilized anti-NK1.1 triggers only a subpopulation of NK cells to kill

Wed Apr 01 00:00:00 UTC 1998

Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen.

Hybridoma

Sentman CL,Hackett J,Moore TA,Tutt MM,Bennett M,Kumar V

MA5-16916 was used in flow cytometry to describe three new NK specific monoclonal antibodies

Fri Dec 01 00:00:00 UTC 1989

Bioinformatics

Protein Aliases: CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; Killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; KLRB1; Ly-55; ly-55b; Ly-55c; Ly-55d; Ly55; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; Lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein NKR-P1B allele B6; natural killer cell surface protein NKR-P1B allele SJL/BALB; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; NK1.1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKR-P1C; NKR-P1D

View more View less

Gene Aliases: AI462337; CD161; Klrb1b; Klrb1c; Klrb1d; ly-55c; Ly-59; Ly55b; Ly55c; Ly55d; Ly59; Nk-1; Nk-1.2; NK-RP1; Nk1; NK1.1; Nk1.2; NKR-P1.9; NKR-P1B; NKR-P1D; NKRP1; Nkrp1-b; NKRP140; Nkrp1b; Nkrp1c; Nkrp1d

View more View less

UniProt ID: (Mouse) Q99JB4, (Mouse) P27814

View more View less

Entrez Gene ID: (Mouse) 80782, (Mouse) 17059

View more View less

Function(s)

receptor activity protein binding carbohydrate binding protein homodimerization activity

Process(es)

natural killer cell activation positive regulation of natural killer cell mediated cytotoxicity negative regulation of natural killer cell mediated cytotoxicity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NK1.1 Monoclonal Antibody (PK136)

Product Details

MA5-16916

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Functional Assay (Functional)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!