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Invitrogen
NKCC1 Monoclonal Antibody (6G7D2)
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FIGURE: 1 / 7
NKCC1 Antibody (MA5-49260) in IHC (P)
Immunohistochemistry analysis of NKCC1 in paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with NKCC1 Monoclonal antibody (Product # MA5-49260) using a dilution of 5 μg/mL overnight at 4°C. Biotin conjugated goat ant... View More
Product Details
MA5-49260
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
6G7D2
Immunogen
E.coli-derived human NKCC1/SLC12A2 recombinant protein (Position: K889-K943).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074918
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human CACO-2 whole cell, human PC-3 whole cell. IHC: human colorectal adenocarcinoma tissue, mouse colon tissue, rat colon tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
NKCC1 mediates sodium and chloride transport and reabsorption. NKCC1 is a membrane protein and is important in maintaining proper ionic balance and cell volume. NKCC1 is an electrically silent transporter system that mediates sodium and chloride reabsorption. Further, NKCC1 plays a vital role in the regulation of ionic balance and cell volume and is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for NKCC1. Diseases associated with SLC12A2 include Basosquamous Carcinoma and Metatypical Basal Cell Carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basolateral Na-K-Cl symporter; BSC2; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; hNKCC1; mNKCC1; Na-K-2Cl cotransporter 1; protein phosphatase 1, regulatory subunit 141; sodium/potassium/chloride cotransporters; sodium/potassium/chloride transporters; solute carrier family 12 (sodium/potassium/chloride transporter), member 2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; Solute carrier family 12 member 2; solute carrier family 12, member 2
Gene Aliases: 9330166H04Rik; BSC; BSC2; mBSC2; NKCC1; PPP1R141; SLC12A2; sy-ns
UniProt ID: (Human) P55011, (Mouse) P55012
Entrez Gene ID: (Human) 6558, (Rat) 83629, (Mouse) 20496
transport
ion transport
potassium ion transport
sodium ion transport
ammonium transport
transepithelial chloride transport
transepithelial ammonium transport
ammonium transmembrane transport
chloride transmembrane transport
chloride transport
hyperosmotic response
gamma-aminobutyric acid signaling pathway
aging
multicellular organism growth
positive regulation of cell volume
detection of mechanical stimulus involved in sensory perception of sound
branching involved in mammary gland duct morphogenesis
mammary duct terminal end bud growth
organic cation transport
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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