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NLE1 Antibody (A305-717A-T) in WB
Detection of human and mouse NLE1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NLE1 antibody A305-717A-M (lot A305-717A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A305-717A-T
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 100 to 150 of human Notchless protein homolog 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Predicted reactivity by homology: Orangutan, Bovine.
Target Information
NLE1 plays a role in regulating Notch activity, in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. NLE1 is required during embryogenesis for inner mass cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ10458; Notchless gene homolog; Notchless protein homolog 1; novel WD40 repeat containing protein
Gene Aliases: AL022765; BC018399; HUSSY-07; l11Jus1; l11Jus4; Nle; NLE1
UniProt ID: (Human) Q9NVX2, (Mouse) Q8VEJ4
Entrez Gene ID: (Human) 54475, (Mouse) 217011
somitogenesis
kidney development
inner cell mass cell differentiation
Notch signaling pathway
ribosomal large subunit biogenesis
negative regulation of mitotic cell cycle
skeletal system morphogenesis
hematopoietic stem cell homeostasis
positive regulation of canonical Wnt signaling pathway
negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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