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Invitrogen
NLGN4X (Neuroligin-4) Monoclonal Antibody (CSTEM30), eBioscience™
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FIGURE: 1 / 2
NLGN4X (Neuroligin-4) Antibody (14-9158-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control (Product # 14-4724-82) (left) or 5 µg/mL NLGN4X (Neuroligin-4) Monoclonal Antibody (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI. An iPSC colony (positive staining) and mouse embryoni... View More
Product Details
14-9158-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
CSTEM30
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with proprietary stabilizer
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2716939
Product Specific Information
This monoclonal antibody CSTEM30 reacts with human Neuroligin-4, X linked, also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X.
Applications Reported: This CSTEM30 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. (Fluorochrome-conjugated CSTEM30 is recommended for use in flow cytometery.)
Applications Tested: This CSTEM30 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human iPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.5 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Target Information
Neuroligin-4, X linked (also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X) is a postsynaptic cell-adhesion molecule that interacts with presynaptic cell-surface neurexins. These interactions bridge together the synaptic cleft and have an essential role in synaptic transmissions between neurons. There are 5 neuroligin genes in humans: NLGN1, NLGN2, NLGN3, NLGN4X, and NLGN4Y. Mutations in NLGN3 and NLGN4X are linked to Autism Spectrum Disorders (ASD). The expression of neuroligin-4, X-linked has been reported on human pluripotent stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HNLX; MGC22376; Neuroligin X; Neuroligin-4, X-linked; NLGN
Gene Aliases: ASPGX2; AUTSX2; HLNX; HNL4X; KIAA1260; NLGN4; NLGN4X; UNQ365/PRO701
UniProt ID: (Human) Q8N0W4
Entrez Gene ID: (Human) 57502
brainstem development
neuron cell-cell adhesion
learning
cerebellum development
neuron differentiation
adult behavior
social behavior
organ growth
cell-cell junction organization
synapse organization
vocalization behavior
negative regulation of excitatory postsynaptic potential
presynaptic membrane assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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