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NLK Antibody (PA5-102708) in ICC/IF
Immunofluorescent analysis of NLK in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and NLK polyclonal antibody (Product # PA5-102708) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102708
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NLK(Accession Q9UBE8), corresponding to amino acid residues H67-A117.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852102
Product Specific Information
Antibody detects endogenous levels of total NLK.
Target Information
NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nemo-like kinase; nlk; Protein LAK1; serine/threonine protein kinase NLK; Serine/threonine-protein kinase NLK
Gene Aliases: AI194375; LAK1; NLK; RGD1561602
UniProt ID: (Human) Q9UBE8, (Mouse) O54949, (Rat) D3ZSZ3
Entrez Gene ID: (Human) 51701, (Mouse) 18099, (Rat) 497961
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
transcription factor binding
ubiquitin protein ligase binding
SH2 domain binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
MAPK cascade
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
transforming growth factor beta receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
peptidyl-threonine phosphorylation
negative regulation of Wnt signaling pathway
serine phosphorylation of STAT3 protein
intracellular signal transduction
protein autophosphorylation
protein stabilization
Wnt signaling pathway
phosphorylation
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