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Product Details
PA5-21877
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NLK. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152101
Product Specific Information
Recommended positive controls: NLK-transfected 293T, A431, H1299, HeLa, mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nemo-like kinase; nlk; Protein LAK1; serine/threonine protein kinase NLK; Serine/threonine-protein kinase NLK
Gene Aliases: AI194375; LAK1; NLK
UniProt ID: (Human) Q9UBE8, (Mouse) O54949
Entrez Gene ID: (Human) 51701, (Mouse) 18099
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
transcription factor binding
ubiquitin protein ligase binding
SH2 domain binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
MAPK cascade
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
transforming growth factor beta receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
peptidyl-threonine phosphorylation
negative regulation of Wnt signaling pathway
serine phosphorylation of STAT3 protein
intracellular signal transduction
protein autophosphorylation
protein stabilization
Wnt signaling pathway
phosphorylation
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