Invitrogen

NLRP3 Recombinant Rabbit Monoclonal Antibody (SC06-23)

3 Published Figures
7 References
View all (29) NLRP3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32255

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
View 2 publications 2 publications

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC06-23

Immunogen

Recombinant protein within Human NLRP3 aa 5-161
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809541

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; cold autoinflammatory syndrome 1 homolog; cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1 protein homolog; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; FLJ95925; mast cell maturation inducible protein 1; Mast cell maturation-associated-inducible protein 1; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NACHT/LRR/pyrin domain-containing protein 3; NLR family, pyrin domain containing 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYRIN-containing APAF1-like protein 1

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Gene Aliases: AGTAVPRL; AII; AII/AVP; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; Mmig1; MWS; NALP3; NLRP3; PYPAF1

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UniProt ID: (Human) Q96P20, (Rat) D4A523, (Mouse) Q8R4B8

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Entrez Gene ID: (Human) 114548, (Rat) 287362, (Mouse) 216799

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Function(s)
protein binding ATP binding transcription factor binding peptidoglycan binding sequence-specific DNA binding molecular_function nucleotide binding
Process(es)
negative regulation of acute inflammatory response positive regulation of type 2 immune response transcription, DNA-templated apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process defense response inflammatory response signal transduction detection of biotic stimulus negative regulation of NF-kappaB transcription factor activity interleukin-1 beta production interleukin-18 production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production negative regulation of NF-kappaB import into nucleus positive regulation of cysteine-type endopeptidase activity involved in apoptotic process NLRP3 inflammasome complex assembly innate immune response positive regulation of T-helper 2 cell differentiation positive regulation of transcription from RNA polymerase II promoter interleukin-1 secretion negative regulation of interleukin-1 beta secretion positive regulation of interleukin-1 beta secretion negative regulation of inflammatory response positive regulation of NF-kappaB transcription factor activity protein oligomerization defense response to virus cellular response to lipopolysaccharide positive regulation of T-helper 17 cell differentiation positive regulation of T-helper 2 cell cytokine production biological_process regulation of inflammatory response
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