Invitrogen

NMDAR1 Polyclonal Antibody

11 Published Figures

24 References

View all (38) NMDAR1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (11)

NMDAR1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

NMDAR1 Antibody (PA3-102) in ICC/IF

Immunofluorescence analysis of NMDA Receptor 1 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NMDA Receptor 1 Rabbit Polyclonal Antibody (Product # PA3-102) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 c... View More

NMDAR1 Antibody in Immunohistochemistry (IHC)

NMDAR1 Antibody in Flow Cytometry (Flow)

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Product Details

PA3-102

Applications

Tested Dilution

Publications

Western Blot (WB)

1:600

View 13 publications 13 publications

Immunohistochemistry (IHC)

1:200

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

View 4 publications 4 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Affinity Purification (AP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Guinea pig, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1 (Isoforms B, F, G).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2112003

Product Specific Information

PA3-102 detects N-methyl-D-aspartate (NMDA) receptor type 1.

PA3-102 has been used successfully in Western blot, ELISA, immunoprecipitation, immunohistochemistry, and immunocytochemistry procedures. In Western blot analysis of rat brain synaptic membranes this antibody detects a ~120 kDa protein representing NMDA receptor type 1.

The PA3-102 immunogen is a synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1 (Isoforms B, F, G).

Target Information

NMDAR1 encodes a protein that is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An LRP1-binding motif in cellular prion protein replicates cell-signaling activities of the full-length protein.

JCI insight

Mantuano E,Zampieri C,Azmoon P,Gunner CB,Heye KR,Gonias SL

PA3-102 was used in Flow Cytometry to show that biological activities of shed PrPC, attributed to interaction with LRP1, are retained in synthetic peptides, which may be templates for therapeutics development.

Tue Aug 08 00:00:00 UTC 2023

An LRP1-binding motif in cellular prion protein replicates cell-signaling activities of the full-length protein.

JCI insight

Mantuano E,Zampieri C,Azmoon P,Gunner CB,Heye KR,Gonias SL

Tue Aug 08 00:00:00 UTC 2023

N-methyl-D-aspartate receptors induce M1 polarization of macrophages: Feasibility of targeted imaging in inflammatory response in vivo.

Cell & bioscience

Jeon HJ,Byun JK,Lee SB,Son KH,Lim JY,Lee DS,Kim KS,Park JW,Shin GR,Kim YJ,Jin J,Kim D,Kim DH,Yu JH,Choi YK,Park KG,Jeon YH

PA3-102 was used in Conjugation experiments to suggest that NMDAR targeting imaging probe may be useful in research on inflammatory response in vivo.

Thu Mar 30 00:00:00 UTC 2023

N-methyl-D-aspartate receptors induce M1 polarization of macrophages: Feasibility of targeted imaging in inflammatory response in vivo.

Cell & bioscience

Jeon HJ,Byun JK,Lee SB,Son KH,Lim JY,Lee DS,Kim KS,Park JW,Shin GR,Kim YJ,Jin J,Kim D,Kim DH,Yu JH,Choi YK,Park KG,Jeon YH

PA3-102 was used in Conjugation experiments to suggest that NMDAR targeting imaging probe may be useful in research on inflammatory response in vivo.

Thu Mar 30 00:00:00 UTC 2023

Antibody induced seizure susceptibility and impaired cognitive performance in a passive transfer rat model of autoimmune encephalitis.

Frontiers in immunology

Pişkin ŞA,Korkmaz HY,Ulusoy CA,Şanlı E,Küçükali CI,Onat F,Tüzün E,Çarçak N

PA3-102 was used in Immunohistochemistry to suggest that neuronal surface auto-antibody administration induces seizure susceptibility and disturbed cognitive performance in the passive transfer rat model of LGI1 AE, which could be a potential in-vivo model for understanding immune-mediated mechanisms underlying epileptogenesis and highlight the potential targets for immune-mediated seizures in AE patients.

Mon Dec 04 00:00:00 UTC 2023

Antibody induced seizure susceptibility and impaired cognitive performance in a passive transfer rat model of autoimmune encephalitis.

Frontiers in immunology

Pişkin ŞA,Korkmaz HY,Ulusoy CA,Şanlı E,Küçükali CI,Onat F,Tüzün E,Çarçak N

Mon Dec 04 00:00:00 UTC 2023

Electroacupuncture modulates glutamate neurotransmission to alleviate PTSD-like behaviors in a PTSD animal model.

Translational psychiatry

Cai M,Park HR,Yang EJ

PA3-102 was used in Western Blot to investigate the effects of electroacupuncture at the GV29 (Yintang) acupoint three times per week for 2 weeks in an animal model of Post-traumatic stress disorder (PTSD).

Wed Nov 22 00:00:00 UTC 2023

Blockage of neuromuscular glutamate receptors impairs reinnervation following nerve crush in adult mice.

Frontiers in cellular neuroscience

Personius KE,Siebert D,Koch DW,Udin SB

PA3-102 was used in Immunohistochemistry to investigate the role of glutamate receptors in motor axons regeneration following injury.

Tue Oct 11 00:00:00 UTC 2022

Blockage of neuromuscular glutamate receptors impairs reinnervation following nerve crush in adult mice.

Frontiers in cellular neuroscience

Personius KE,Siebert D,Koch DW,Udin SB

PA3-102 was used in Immunohistochemistry to investigate the role of glutamate receptors in motor axons regeneration following injury.

Tue Oct 11 00:00:00 UTC 2022

p140Cap Regulates the Composition and Localization of the NMDAR Complex in Synaptic Lipid Rafts.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Angelini C,Morellato A,Alfieri A,Pavinato L,Cravero T,Bianciotto OT,Salemme V,Natalini D,Centonze G,Raspanti A,Garofalo T,Valdembri D,Serini G,Marcantoni A,Becchetti A,Giustetto M,Turco E,Defilippi P

PA3-102 was used in Western Blotting to provide the molecular and functional dissection of p140Cap as a new active member of a highly dynamic synaptic network involved in memory consolidation, LTP and LTD that are known to be altered in neurological and psychiatric disorders.

Wed Sep 21 00:00:00 UTC 2022

Autism-Misregulated eIF4G Microexons Control Synaptic Translation and Higher Order Cognitive Functions.

Molecular cell

Gonatopoulos-Pournatzis T,Niibori R,Salter EW,Weatheritt RJ,Tsang B,Farhangmehr S,Liang X,Braunschweig U,Roth J,Zhang S,Henderson T,Sharma E,Quesnel-Vallières M,Permanyer J,Maier S,Georgiou J,Irimia M,Sonenberg N,Forman-Kay JD,Gingras AC,Collingridge GL,Woodin MA,Cordes SP,Blencowe BJ

PA3-102 was used in Western Blotting to reveal an autism-disrupted mechanism by which alternative splicing specializes neuronal translation to control higher order cognitive functioning.

Thu Mar 19 00:00:00 UTC 2020

Glycogen synthase kinase-3 inhibition rescues sex-dependent contextual fear memory deficit in human immunodeficiency virus-1 transgenic mice.

British journal of pharmacology

Moidunny S,Benneyworth MA,Titus DJ,Beurel E,Kolli U,Meints J,Jalodia R,Ramakrishnan S,Atkins CM,Roy S

PA3-102 was used in Western Blot to investigate synaptic mechanisms associated with sex differences in HIV-associated neurocognitive disorders.

Tue Dec 01 00:00:00 UTC 2020

Repeated Galvanic Vestibular Stimulation Modified the Neuronal Potential in the Vestibular Nucleus.

Neural plasticity

Kim G,Lee S,Kim KS

PA3-102 was used in Immunohistochemistry to show that AMPA and NMDA receptors decreased after the repeated stimuli by 28.32 and 16.09%, respectively, implying the modification in the neuronal amplitudes.

Wed Apr 07 00:00:00 UTC 2021

Synaptic proximity enables NMDAR signalling to promote brain metastasis.

Nature

Zeng Q,Michael IP,Zhang P,Saghafinia S,Knott G,Jiao W,McCabe BD,Galván JA,Robinson HPC,Zlobec I,Ciriello G,Hanahan D

PA3-102 was used in Immunohistochemistry to show that B2BM cells co-opt a neuronal signalling pathway that was recently implicated in invasive tumour growth, involving activation by glutamate ligands of N-methyl-D-aspartate receptors (NMDARs), which is key in model systems for metastatic colonization of the brain and is associated with poor prognosis.

Sun Sep 01 00:00:00 UTC 2019

Hydrogen sulfide inhibits Ca²⁺-induced mitochondrial permeability transition pore opening in type-1 diabetes.

American journal of physiology. Endocrinology and metabolism

Papu John AS,Kundu S,Pushpakumar S,Amin M,Tyagi SC,Sen U

PA3-102 was used in Western Blot to show that NMDA-R1-mediated Ca2+ influx in diabetes induces MPTP opening via CypD activation leading to increased oxidative stress and renal injury, and H2S protects diabetic kidney from injury by blocking mitochondrial Ca2+ permeability through NMDA-R1 pathway.

Thu Aug 01 00:00:00 UTC 2019

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Sun Apr 21 00:00:00 UTC 2024

Tissue-type plasminogen activator selectively inhibits multiple toll-like receptors in CSF-1-differentiated macrophages.

PloS one

Das L,Azmoon P,Banki MA,Mantuano E,Gonias SL

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Flow Cytometry

Wed Mar 18 00:00:00 UTC 2020

Red and near-infrared light induces intracellular Ca²⁺ flux via the activation of glutamate N-methyl-D-aspartate receptors.

Journal of cellular physiology

Golovynska I,Golovynskyi S,Stepanov YV,Garmanchuk LV,Stepanova LI,Qu J,Ohulchanskyy TY

PA3-102 was used in Immunohistochemistry-immunofluorescence to demonstrate that NIR light can be used for nonthermal and nonpharmacological stimulation of NMDARs in cancer cells.

Sun Sep 01 00:00:00 UTC 2019

Cereblon Maintains Synaptic and Cognitive Function by Regulating BK Channel.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Choi TY,Lee SH,Kim YJ,Bae JR,Lee KM,Jo Y,Kim SJ,Lee AR,Choi S,Choi LM,Bang S,Song MR,Chung J,Lee KJ,Kim SH,Park CS,Choi SY

PA3-102 was used in Western Blotting to investigate the role of cereblon in synaptic function and animal behaviour.

Wed Apr 04 00:00:00 UTC 2018

Upregulated SK2 Expression and Impaired CaMKII Phosphorylation Are Shared Synaptic Defects Between 16p11.2del and 129S:Δdisc1 Mutant Mice.

ASN neuro

Sultana R,Ghandi T,M Davila A,Lee CC,Ogundele OM

PA3-102 was used in Western Blotting to demonstrate that the expression of small-conductance potassium channels (SK2 or KCa2.2) was significantly upregulated in the hippocampus and prefrontal cortex of 16p11.2del and 129S:Δdisc1 mutant mice.

Mon Apr 08 00:00:00 UTC 2024

Autism-like behavior caused by deletion of vaccinia-related kinase 3 is improved by TrkB stimulation.

The Journal of experimental medicine

Kang MS,Choi TY,Ryu HG,Lee D,Lee SH,Choi SY,Kim KT

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Mon Oct 02 00:00:00 UTC 2017

Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Frontiers in cellular neuroscience

Lisek M,Ferenc B,Studzian M,Pulaski L,Guo F,Zylinska L,Boczek T

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Thu Oct 01 00:00:00 UTC 2020

Low levels of methyl β-cyclodextrin disrupt GluA1-dependent synaptic potentiation but not synaptic depression.

Journal of neurochemistry

Choi TY,Jung S,Nah J,Ko HY,Jo SH,Chung G,Park K,Jung YK,Choi SY

PA3-102 was used in western blot to study the effects of methyl beta-cyclodextrin on GluA1-dependent synaptic potentiation

Sun Feb 01 00:00:00 UTC 2015

Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Portugal GS,Al-Hasani R,Fakira AK,Gonzalez-Romero JL,Melyan Z,McCall JG,Bruchas MR,Morón JA

PA3-102 was used in western blot to study the reinstatement of morphine conditioned place preference and the role of hippocampal synaptic plasticity and NMDAR expression

Wed Jan 08 00:00:00 UTC 2014

Sox18 preserves the pulmonary endothelial barrier under conditions of increased shear stress.

Journal of cellular physiology

Gross CM,Aggarwal S,Kumar S,Tian J,Kasa A,Bogatcheva N,Datar SA,Verin AD,Fineman JR,Black SM

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Sat Nov 01 00:00:00 UTC 2014

Hijacking the neuronal NMDAR signaling circuit to promote tumor growth and invasion.

Cell

Li L,Hanahan D

PA3-102 was used in flow cytometry and immunohistochemistry to study the role of NMDAR signaling in tumorigenesis using a murine model

Thu Mar 28 00:00:00 UTC 2013

Impacts of forebrain neuronal glycine transporter 1 disruption in the senescent brain: evidence for age-dependent phenotypes in Pavlovian learning.

Behavioral neuroscience

Dubroqua S,Singer P,Boison D,Feldon J,Möhler H,Yee BK

PA3-102 was used in immunohistochemistry to investigate the role of forebrain neuronal glycine transporter 1 in cognitive functions

Wed Dec 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: GluN1; Glun1r; glutamate [NMDA] receptor subunit zeta 1; Glutamate [NMDA] receptor subunit zeta-1; glutamate receptor; Glutamate receptor ionotropic, NMDA 1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; N-methyl-D-aspartate glutamate receptor; N-methyl-D-aspartate receptor channel, subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; neurotransmitter receptor; NMD-R1; NMDA R1 receptor C1 cassette; NMDA receptor 1; NMDZ1; NMZ1; NR1C1; NR1C2; NR1C2'; NR1N1; pGluN1; RP11-350O14.1

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Gene Aliases: GluN1; GluRdelta1; Glurz1; GluRzeta1; GRIN1; M100174; MRD8; NMD-R1; NMDA1; Nmdar; NMDAR1; NR1; Rgsc174

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UniProt ID: (Human) Q05586, (Rat) P35439, (Mouse) P35438

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Entrez Gene ID: (Human) 2902, (Rat) 24408, (Mouse) 14810

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Function(s)

NMDA glutamate receptor activity Ras guanyl-nucleotide exchange factor activity extracellular-glutamate-gated ion channel activity calcium channel activity calcium ion binding protein binding calmodulin binding glycine binding glutamate binding enzyme binding voltage-gated cation channel activity glutamate receptor binding neurotransmitter binding protein heterodimerization activity ionotropic glutamate receptor activity receptor binding cation channel activity protein dimerization activity receptor activity ion channel activity

Process(es)

MAPK cascade conditioned taste aversion suckling behavior response to amphetamine cation transport cellular calcium ion homeostasis chemical synaptic transmission respiratory gaseous exchange long-term memory adult locomotory behavior olfactory learning visual learning propylene metabolic process sensory perception of pain pons maturation cerebral cortex development regulation of ion transmembrane transport social behavior ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic regulation of membrane potential positive regulation of apoptotic process response to morphine negative regulation of neuron apoptotic process positive regulation of GTPase activity regulation of respiratory gaseous exchange response to ethanol positive regulation of transcription from RNA polymerase II promoter ephrin receptor signaling pathway regulation of long-term neuronal synaptic plasticity rhythmic process regulation of dendrite morphogenesis regulation of axonogenesis protein tetramerization response to calcium ion regulation of synapse assembly calcium ion homeostasis excitatory postsynaptic potential prepulse inhibition male mating behavior response to fungicide calcium ion transmembrane transport cellular response to manganese ion positive regulation of excitatory postsynaptic potential startle response calcium ion transport learning or memory learning memory associative learning regulation of cell communication positive regulation of cell death response to organic cyclic compound response to amine ion transmembrane transport regulation of neuron apoptotic process regulation of synaptic plasticity regulation of neuronal synaptic plasticity neuromuscular process transport ion transport cation transmembrane transport

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NMDAR1 Polyclonal Antibody

NMDAR1 Antibody (PA3-102) in ICC/IF

Product Details

PA3-102

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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