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Invitrogen
NMDAR2B Polyclonal Antibody
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FIGURE: 1 / 3
NMDAR2B Antibody (PA5-18536) in ICC/IF
Immunocytochemistry analysis of NMDAR2B using NMDAR2B Polyclonal Antibody (Product # PA5-18536) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (KDAHEKDDFHHLS) corresponding to the internal amino acids of GRIN2B (aa 51-64).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10985830
Product Specific Information
This antibody is predicted to react with canine, human and mouse based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; GRIN 2B; HGNC:4586; hNR 3; MGC142178; MGC142180; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDAR2B; NMDE2; NME2; NR2B; NR3
Gene Aliases: EIEE27; GluN2B; GRIN2B; hNR3; MRD6; NMDAR2B; NR2B
UniProt ID: (Human) Q13224, (Rat) Q00960
Entrez Gene ID: (Human) 2904, (Rat) 24410
NMDA glutamate receptor activity
Ras guanyl-nucleotide exchange factor activity
extracellular-glutamate-gated ion channel activity
protein binding
zinc ion binding
glycine binding
ionotropic glutamate receptor activity
receptor binding
interleukin-1 receptor binding
cation channel activity
calcium channel activity
beta-catenin binding
drug binding
D2 dopamine receptor binding
ionotropic glutamate receptor binding
neurotransmitter binding
protein heterodimerization activity
cell adhesion molecule binding
MAPK cascade
transport
glutamate receptor signaling pathway
chemical synaptic transmission
learning or memory
ion transmembrane transport
ionotropic glutamate receptor signaling pathway
positive regulation of GTPase activity
response to ethanol
ephrin receptor signaling pathway
action potential
behavioral fear response
in utero embryonic development
startle response
suckling behavior
response to amphetamine
cation transport
calcium ion transport
sensory organ development
learning
memory
long-term memory
associative learning
response to mechanical stimulus
response to toxic substance
response to carbohydrate
response to manganese ion
cellular response to magnesium starvation
regulation of protein kinase A signaling
positive regulation of cell death
positive regulation of glutamate secretion
response to organic cyclic compound
response to amine
hippocampus development
cerebral cortex development
response to magnesium ion
multicellular organismal response to stress
response to cytokine
synaptic transmission, glutamatergic
response to cocaine
fear response
receptor clustering
regulation of MAPK cascade
regulation of synaptic plasticity
regulation of long-term neuronal synaptic plasticity
behavioral response to pain
rhythmic process
positive regulation of synaptic transmission
detection of mechanical stimulus involved in sensory perception of pain
response to calcium ion
response to methylmercury
response to electrical stimulus
response to other organism
regulation of postsynaptic membrane potential
excitatory postsynaptic potential
long-term synaptic potentiation
response to growth hormone
response to fungicide
calcium ion transmembrane transport
cellular response to amino acid stimulus
cellular response to manganese ion
cellular response to dsRNA
cellular response to growth factor stimulus
cellular response to lipid
cellular response to organic cyclic compound
cation transmembrane transport
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